Document Detail

Zinc salts inhibit in vitro Toll-like receptor 2 surface expression by keratinocytes.
MedLine Citation:
PMID:  17951128     Owner:  NLM     Status:  MEDLINE    
Propionibacterium acnes (P. acnes) plays an important role in the induction and maintenance of the inflammatory phase of acne. At the therapeutic level, it has been shown that zinc salts could have a beneficial effect on mild and moderate inflammatory acne lesions. However, their mechanisms of action are still only partially known. Immediate early immune response is a crucial route in the development of inflammatory reaction and, specifically, activation of Toll-like Receptors (TLRs) leading to nuclear factor (NF)-kappaB translocation and production of inflammatory cytokines such as interleukin-8 (IL-8). The aim of this work was to determine if cytokine secretion and innate immunity could be targets of zinc salts. Normal Human Epidermal Keratinocytes (NHEK) and skin explants were stimulated by P. acnes extracts and incubated (3 h) with zinc salts (1 microg/mL). Then we successively studied TLR2 expression by immunohistochemistry and IL-8 production by ELISA. After incubation with zinc salts, the increase of TLR2 surface expression in skin upon membrane fraction (FM) of P. acnes challenge was decreased as compared to that in control samples. However, this inhibition does not modify IL-8 secretion by keratinocytes. In conclusion the inhibition of TLR2 surface expression by keratinocytes could be one of the anti-inflammatory mechanisms of zinc salts in acne.
Véronique Jarrousse; Nathalie Castex-Rizzi; Amir Khammari; Marie Charveron; Brigitte Dréno
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-19
Journal Detail:
Title:  European journal of dermatology : EJD     Volume:  17     ISSN:  1167-1122     ISO Abbreviation:  Eur J Dermatol     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-02-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206420     Medline TA:  Eur J Dermatol     Country:  France    
Other Details:
Languages:  eng     Pagination:  492-6     Citation Subset:  IM    
INSERM U601, 9, quai Moncousu, 44093, Nantes Cedex 01, France.
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MeSH Terms
Cell Extracts / pharmacology
Cells, Cultured
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Interleukin-8 / metabolism*
Keratinocytes / drug effects*,  immunology,  metabolism
Lipopolysaccharides / pharmacology
Organ Culture Techniques
Propionibacterium acnes / immunology
Skin / drug effects*,  immunology,  metabolism
Toll-Like Receptor 2 / metabolism*
Zinc / pharmacology*
Reg. No./Substance:
0/Cell Extracts; 0/Interleukin-8; 0/Lipopolysaccharides; 0/Toll-Like Receptor 2; 7440-66-6/Zinc

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