Document Detail


Zinc improves the filterability of sickle erythrocytes at intermediate oxygen partial pressures.
MedLine Citation:
PMID:  1655346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The deformability of erythrocytes from patients with sickle-cell anaemia was measured with a St George's blood filtrometer at a range of oxygen partial pressures and at four levels of zinc loading. 2. When incubated in buffered saline containing zinc and the chelator ethyl maltol, erythrocytes rapidly accumulated zinc and thus their oxygen affinity was increased. 3. Neither the oxygen partial pressure nor zinc loading affected the filtration of normal erythrocytes. 4. Deoxygenation of sickle erythrocytes greatly impaired filtration, although the initial filtration rate declined sharply at different oxygen partial pressures (between 70 and 35 mmHg) in different patients. 5. Low levels of zinc (0.03 +/- 0.003 mol of zinc/mol of haemoglobin tetramer) were without effect on sickle cells, but at zinc/haemoglobin ratios of 0.6:1 and above, the sharp fall in filtration rate occurred at oxygen partial pressures 8-25 mmHg below the oxygen partial pressure that impaired filtration of untreated cells. 6. Hence, the deformability of sickle erythrocytes in vitro can be improved by increasing the intracellular content of zinc to 20-fold above normal. Further studies are now required to examine the stability of zinc in erythrocytes, the effects of high intracellular zinc concentrations on erythrocyte viability, and the toxicity of zinc released from zinc-laden cells.
Authors:
J A Taylor; J Acharya; T C Pearson; R P Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  81     ISSN:  0143-5221     ISO Abbreviation:  Clin. Sci.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-11-18     Completed Date:  1991-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  433-8     Citation Subset:  IM    
Affiliation:
Gastrointestinal Laboratory, Rayne Institute, St Thomas' Hospital, London.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Erythrocyte Deformability / drug effects*
Erythrocytes / metabolism
Hemofiltration / methods
Humans
Oxygen / blood*
Sickle Cell Trait / blood*
Zinc / metabolism*,  pharmacology
Chemical
Reg. No./Substance:
7440-66-6/Zinc; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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