Document Detail

Zebrafish small molecule screen in reprogramming/cell fate modulation.
MedLine Citation:
PMID:  20336532     Owner:  NLM     Status:  MEDLINE    
Embryonic zebrafish have long been used for lineage-tracing studies. In zebrafish embryos, the cell fate identities can be determined by whole-mount in situ hybridization, or by visualization of live embryos if using fluorescent reporter lines. We use embryonic zebrafish to study the effects of a leukemic oncogene AML1-ETO on modulating hematopoietic cell fate. Induced expression of AML1-ETO is able to efficiently reprogram hematopoietic progenitor cells from erythroid to myeloid cell fate. Using the zebrafish model of AML1-ETO, we performed a chemical screen to identify small molecules that suppress the cell fate switch in the presence of AML1-ETO. The methods discussed herein may be broadly applicable for identifying small molecules that modulate other cell fate decisions.
Jing-Ruey J Yeh; Kathleen M Munson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  636     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-07     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-27     Citation Subset:  IM    
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MeSH Terms
Animals, Genetically Modified
Cell Lineage
Core Binding Factor Alpha 2 Subunit / genetics,  metabolism
Hematopoietic Stem Cells / cytology,  physiology*
In Situ Hybridization / methods
Nuclear Reprogramming*
Oncogene Proteins, Fusion / genetics,  metabolism
Small Molecule Libraries*
Zebrafish* / anatomy & histology,  embryology
Grant Support
AG031300/AG/NIA NIH HHS; K01 AG031300/AG/NIA NIH HHS; K01 AG031300-01A1/AG/NIA NIH HHS; K01 AG031300-02/AG/NIA NIH HHS; K01 AG031300-03/AG/NIA NIH HHS; R01 CA118498/CA/NCI NIH HHS; R01 CA140188/CA/NCI NIH HHS
Reg. No./Substance:
0/Core Binding Factor Alpha 2 Subunit; 0/Oncogene Proteins, Fusion; 0/Small Molecule Libraries

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