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Zebrafish mast cells possess an FcɛRI-like receptor and participate in innate and adaptive immune responses.
MedLine Citation:
PMID:  20849876     Owner:  NLM     Status:  In-Process    
We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p=0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p=0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (FcɛRIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish FcɛRI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p=0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.
Sahar Da'as; Evelyn M Teh; J Tristan Dobson; Gheyath K Nasrallah; Eileen R McBride; Hao Wang; Donna S Neuberg; Jean S Marshall; Tong-Jun Lin; Jason N Berman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-19
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  35     ISSN:  1879-0089     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-10-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
IWK Health Centre, Dalhousie University, PO Box 9700, 5850/5980 University Avenue, Halifax, NS B3K 6R8, Canada.
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Grant Support
//Canadian Institutes of Health Research

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