| Zebrafish cdc25a is expressed during early development and limiting for post-blastoderm cell cycle progression. | |
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MedLine Citation:
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PMID: 17969147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cdc25 phosphatases are required for eukaryotic cell cycle progression. To investigate mechanisms governing spatiotemporal dynamics of cell cycle progression during vertebrate development, we isolated two cdc25 genes from the zebrafish, Danio rerio, cdc25a, and cdc25d. We propose that Zebrafish cdc25a is the zebrafish orthologue of the tetrapod Cdc25A genes, while cdc25d is of indeterminate origin. We show that both genes have proliferation promoting activity, but that only cdc25d can complement a Schizosaccharomyces pombe loss of function cdc25 mutation. We present expression data demonstrating that cdc25d expression is very limited during early development, while cdc25a is widely expressed and consistent with the mitotic activity in previously identified mitotic domains of the post-blastoderm zebrafish embryo. Finally, we show that cdc25a can accelerate the entry of post-blastoderm cells into mitosis, suggesting that levels of cdc25a are rate limiting for cell cycle progression during gastrulation. |
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Authors:
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Damian E Dalle Nogare; Andrea Arguello; Shelley Sazer; Mary Ellen Lane |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 236 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-26 Completed Date: 2008-02-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 3427-35 Citation Subset: IM |
Copyright Information:
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2007 Wiley-Liss, Inc |
Affiliation:
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Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Cycle / genetics Cloning, Molecular DNA Primers / genetics Gastrulation / genetics Gene Expression Regulation, Developmental Genes, Fungal Genetic Complementation Test Molecular Sequence Data Phylogeny Schizosaccharomyces / cytology, enzymology, genetics Sequence Homology, Amino Acid Zebrafish / embryology*, genetics* cdc25 Phosphatases / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY015305/EY/NEI NIH HHS; R01 EY015305-03/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 3.1.3.48/cdc25 Phosphatases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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