Document Detail

Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling.
MedLine Citation:
PMID:  15707958     Owner:  NLM     Status:  MEDLINE    
We analyzed Polycomb group gene ph2alpha functionally in zebrafish embryos by a gene knock-down procedure using morpholino antisense oligos. Inhibition of ph2alpha message translation resulted in abnormal epibolic movements as well as a thick tailbud or incomplete covering of the yolk plug. At the 24hpf stage, morphants had short trunks and tails, phenotypes similar to those with disturbances in FGF signaling. Accordingly, we looked at the effects of ph2alpha expression upstream and downstream of the FGF pathway. Treatment with SU5402, an inhibitor of Fgfrs, or injection of dominant-negative Fgfr1 DNA markedly reduced ph2alpha expression in the tailbud. In addition, cells expressing mRNAs for no tail, spadetail, myoD, and papc, which are involved in FGF-related development of posterior mesoderm, were distributed abnormally. Collectively, the data argue that ph2alpha is required for epiboly and tailbud formation, acting downstream of the FGF signaling pathway.
Yuta Komoike; Akinori Kawamura; Norihisa Shindo; Chie Sato; Junichi Satoh; Robert Shiurba; Toru Higashinakagawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  328     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-04-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  858-66     Citation Subset:  IM    
Department of Biology, School of Education and in Integrated Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku, Tokyo 169-8050, Japan.
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MeSH Terms
Carrier Proteins / genetics,  metabolism*
Fibroblast Growth Factors / metabolism*
Gene Expression Regulation, Developmental / physiology*
Gene Silencing
Limb Buds
Repressor Proteins / genetics,  metabolism*
Signal Transduction / physiology
Tissue Distribution
Zebrafish / anatomy & histology,  embryology*,  metabolism*
Zebrafish Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Carrier Proteins; 0/Repressor Proteins; 0/Zebrafish Proteins; 0/edr2 protein, zebrafish; 0/polycomb group proteins; 62031-54-3/Fibroblast Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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