Document Detail

Zebrafish (Danio rerio) endomembrane antiporter similar to a yeast cation/H(+) transporter is required for neural crest development.
MedLine Citation:
PMID:  20578725     Owner:  NLM     Status:  MEDLINE    
CAtion/H(+) eXchangers (CAXs) are integral membrane proteins that transport Ca(2+) or other cations by exchange with protons. While several yeast and plant CAX proteins have been characterized, no functional analysis of a vertebrate CAX homologue has yet been reported. In this study, we further characterize a CAX from yeast, VNX1, and initiate characterization of a zebrafish CAX (Cax1). Localization studies indicated that both Vnx1 and Cax1 proteins are found in endomembrane compartments. Biochemical characterization of endomembrane fractions from vnx1 mutant cells and zebrafish Cax1-expressing yeast cells suggested that both yeast and fish CAXs have Ca(2+)/H(+) antiport activities. Additionally, the vnx1 mutation was associated with heightened pH-sensitivity. In zebrafish embryos, cax1 was specifically expressed in neural crest cells. Morpholino knockdown of cax1 caused defects in neural crest development, including alterations in pigmentation, defects in jaw development, and reduction in expression of the neural crest marker, Pax7. Collectively, our findings provide insights into Vnx1 function and support an unexpected role of CAX transporters in animal growth and development.
Murli Manohar; Hui Mei; Andrew J Franklin; Elly M Sweet; Toshiro Shigaki; Bruce B Riley; Colin W Macdiarmid; Kendal Hirschi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  49     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6557-66     Citation Subset:  IM    
Vegetable and Fruit Improvement Center, Texas A&M University, College Station, Texas 77845, USA.
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MeSH Terms
Antiporters / physiology*
Cation Transport Proteins
Embryo, Nonmammalian / chemistry
Fungal Proteins
Hydrogen-Ion Concentration
Intracellular Membranes / chemistry
Membrane Transport Proteins / physiology
Neural Crest / growth & development*
Saccharomyces cerevisiae Proteins
Sodium-Hydrogen Antiporter
Zebrafish Proteins / physiology*
Reg. No./Substance:
0/Antiporters; 0/CAX1 protein, zebrafish; 0/Cation Transport Proteins; 0/Fungal Proteins; 0/Membrane Transport Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Sodium-Hydrogen Antiporter; 0/Vnx1 protein, S cerevisiae; 0/Zebrafish Proteins

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