| Zebrafish (Danio rerio) endomembrane antiporter similar to a yeast cation/H(+) transporter is required for neural crest development. | |
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MedLine Citation:
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PMID: 20578725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CAtion/H(+) eXchangers (CAXs) are integral membrane proteins that transport Ca(2+) or other cations by exchange with protons. While several yeast and plant CAX proteins have been characterized, no functional analysis of a vertebrate CAX homologue has yet been reported. In this study, we further characterize a CAX from yeast, VNX1, and initiate characterization of a zebrafish CAX (Cax1). Localization studies indicated that both Vnx1 and Cax1 proteins are found in endomembrane compartments. Biochemical characterization of endomembrane fractions from vnx1 mutant cells and zebrafish Cax1-expressing yeast cells suggested that both yeast and fish CAXs have Ca(2+)/H(+) antiport activities. Additionally, the vnx1 mutation was associated with heightened pH-sensitivity. In zebrafish embryos, cax1 was specifically expressed in neural crest cells. Morpholino knockdown of cax1 caused defects in neural crest development, including alterations in pigmentation, defects in jaw development, and reduction in expression of the neural crest marker, Pax7. Collectively, our findings provide insights into Vnx1 function and support an unexpected role of CAX transporters in animal growth and development. |
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Authors:
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Murli Manohar; Hui Mei; Andrew J Franklin; Elly M Sweet; Toshiro Shigaki; Bruce B Riley; Colin W Macdiarmid; Kendal Hirschi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 49 ISSN: 1520-4995 ISO Abbreviation: Biochemistry Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-09-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 6557-66 Citation Subset: IM |
Affiliation:
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Vegetable and Fruit Improvement Center, Texas A&M University, College Station, Texas 77845, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antiporters / physiology* Cation Transport Proteins Embryo, Nonmammalian / chemistry Fungal Proteins Hydrogen-Ion Concentration Intracellular Membranes / chemistry Membrane Transport Proteins / physiology Neural Crest / growth & development* Saccharomyces cerevisiae Proteins Sodium-Hydrogen Antiporter Zebrafish* Zebrafish Proteins / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antiporters; 0/CAX1 protein, zebrafish; 0/Cation Transport Proteins; 0/Fungal Proteins; 0/Membrane Transport Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Sodium-Hydrogen Antiporter; 0/Vnx1 protein, S cerevisiae; 0/Zebrafish Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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