Document Detail


Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish.
MedLine Citation:
PMID:  23757414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Advances in imaging and cell-labeling techniques have greatly enhanced our understanding of developmental and neurobiological processes. Among vertebrates, zebrafish is uniquely suited for in vivo imaging owing to its small size and optical translucency. However, distinguishing and following cells over extended time periods remains difficult. Previous studies have demonstrated that Cre recombinase-mediated recombination can lead to combinatorial expression of spectrally distinct fluorescent proteins (RFP, YFP and CFP) in neighboring cells, creating a 'Brainbow' of colors. The random combination of fluorescent proteins provides a way to distinguish adjacent cells, visualize cellular interactions and perform lineage analyses. Here, we describe Zebrabow (Zebrafish Brainbow) tools for in vivo multicolor imaging in zebrafish. First, we show that the broadly expressed ubi:Zebrabow line provides diverse color profiles that can be optimized by modulating Cre activity. Second, we find that colors are inherited equally among daughter cells and remain stable throughout embryonic and larval stages. Third, we show that UAS:Zebrabow lines can be used in combination with Gal4 to generate broad or tissue-specific expression patterns and facilitate tracing of axonal processes. Fourth, we demonstrate that Zebrabow can be used for long-term lineage analysis. Using the cornea as a model system, we provide evidence that embryonic corneal epithelial clones are replaced by large, wedge-shaped clones formed by centripetal expansion of cells from the peripheral cornea. The Zebrabow tool set presented here provides a resource for next-generation color-based anatomical and lineage analyses in zebrafish.
Authors:
Y Albert Pan; Tom Freundlich; Tamily A Weissman; David Schoppik; X Cindy Wang; Steve Zimmerman; Brian Ciruna; Joshua R Sanes; Jeff W Lichtman; Alexander F Schier
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  140     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-12     Completed Date:  2013-08-20     Revised Date:  2013-08-23    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2835-46     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. ypan@gru.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified / embryology,  metabolism
Cell Lineage
Green Fluorescent Proteins / genetics,  metabolism
Integrases / genetics,  metabolism
Zebrafish / embryology*,  metabolism
Grant Support
ID/Acronym/Agency:
R01 HD067140/HD/NICHD NIH HHS; R01 HD067140/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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