| Zearalenone is bioactivated in the river Buffalo (Bubalus bubalis): hepatic biotransformation. | |
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MedLine Citation:
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PMID: 20361255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zearalenone (ZEA) as a mycoestrogen is found frequently in human foods and animal feeds. Its estrogenic effects depend on its biotransformation fate including both first- and second-phase reactions, which are predominantly governed by hydroxylation and glucuronidation, respectively. In this study, we investigate the hepatic biotransformation of ZEA in river buffalo. To evaluate the hepatic biotransformation of ZEA, both subcellular fractions of the liver were prepared. ZEA was incubated with intracellular subfractions in the presence of nicotinamide dinucleotide phosphate, and the products were determined by means of high-performance liquid chromatography. Moreover, in the same frame of experiment and in the presence of uridine diphosphate glucuronic acid, the rate of glucuronidation for substrate and products were estimated as well. We found that alpha-zearalenol (alpha-ZOL) is the major hydroxylated hepatic metabolite of ZEA produced by both studied subcellular fractions. The enzymatic kinetics analyses indicated that the alpha-ZOL and beta-ZOL production by microsomal fraction were two- and three-fold higher than those by postmitochondrial fraction, respectively. The calculated data showed that alpha-ZOL is conjugated with glucuronic acid more than ZEA and beta-ZOL, especially at the lower concentrations, which seems to be more applicable. Our data suggest that unlike other domestic ruminants including cattle and sheep, the hepatic biotransformation of ZEA in river buffalo results in bioactivation and formation of potent estrogenic metabolite. Moreover, at the relevant concentrations, the produced potent estrogenic metabolite is entirely conjugated with glucuronic acid and, consequently, may cause the prolongation of presence of the compound in the body due to enterohepatic cycle. |
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Authors:
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Malekinejad Hassan; Rahmani Fatemeh; Bahrampour Kobra |
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Publication Detail:
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Type: Journal Article Date: 2010-04-02 |
Journal Detail:
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Title: Tropical animal health and production Volume: 42 ISSN: 1573-7438 ISO Abbreviation: Trop Anim Health Prod Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1277355 Medline TA: Trop Anim Health Prod Country: United States |
Other Details:
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Languages: eng Pagination: 1229-34 Citation Subset: IM |
Affiliation:
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Department of Pharmacology & Toxicology, Faculty of Veterinary Medicine, Urmia University, P.O. Box 1177, Urmia, Iran. hassanmalekinejad@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotransformation / physiology Buffaloes / metabolism* Chromatography, High Pressure Liquid Estrogens, Non-Steroidal / metabolism* Kinetics Liver / metabolism* Microsomes / metabolism NADP Uridine Diphosphate Glucuronic Acid Zearalenone / metabolism* Zeranol / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Estrogens, Non-Steroidal; 17924-92-4/Zearalenone; 2616-64-0/Uridine Diphosphate Glucuronic Acid; 26538-44-3/Zeranol; 53-59-8/NADP |
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