Document Detail


ZFX regulates glioma cell proliferation and survival in vitro and in vivo.
MedLine Citation:
PMID:  23322077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zinc finger transcription factor ZFX functions as an important regulator of self-renewal in multiple stem cell types, as well as a sex determinant of mammals. Moreover, ZFX expression is abnormally elevated in several cancers, and correlates with malignancy grade. To investigate its role in the pathogenesis of gliomas, we used lentivirus-mediated RNA interference (RNAi) to knockdown ZFX expression in human glioma cell lines. Our results demonstrate that ZFX plays a crucial role in glioma proliferation and survival, confirming recent reports. We also show for the first time that ZFX knockdown decreases the in vivo growth potential of U87 glioma xenografts in both subcutaneous and intracranial models in nude mice. We conclude that lentivirus-mediated RNAi targeting of ZFX may serve as a promising strategy for glioma therapy.
Authors:
Zhichuan Zhu; Kui Li; Dafeng Xu; Yongjie Liu; Hailiang Tang; Qing Xie; Liqian Xie; Jiwei Liu; Hongtao Wang; Ye Gong; Zelan Hu; Jing Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  112     ISSN:  1573-7373     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-09-05     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging / drug effects
Animals
Antiviral Agents / pharmacology
Brain Neoplasms / genetics*,  pathology*
Cell Cycle / drug effects,  genetics
Cell Line, Tumor
Cell Proliferation* / drug effects
Cell Survival / drug effects,  genetics
Flow Cytometry
Glioma / genetics*,  pathology
Humans
In Situ Nick-End Labeling
Kruppel-Like Transcription Factors / genetics,  metabolism*
Lentivirus / genetics,  physiology
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Mice, Nude
Oncogene Protein v-akt / metabolism
RNA, Small Interfering / pharmacology
Signal Transduction / drug effects
Time Factors
Tumor Stem Cell Assay
Tunicamycin / pharmacology
Up-Regulation / drug effects,  genetics
Xenograft Model Antitumor Assays
beta-Galactosidase / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Kruppel-Like Transcription Factors; 0/RNA, Small Interfering; 0/zinc finger protein, X-linked; 11089-65-9/Tunicamycin; EC 2.7.11.1/Oncogene Protein v-akt; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections
Erratum In:
J Neurooncol. 2013 Jul;113(3):533

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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