|ZFX regulates glioma cell proliferation and survival in vitro and in vivo.|
|PMID: 23322077 Owner: NLM Status: MEDLINE|
|The zinc finger transcription factor ZFX functions as an important regulator of self-renewal in multiple stem cell types, as well as a sex determinant of mammals. Moreover, ZFX expression is abnormally elevated in several cancers, and correlates with malignancy grade. To investigate its role in the pathogenesis of gliomas, we used lentivirus-mediated RNA interference (RNAi) to knockdown ZFX expression in human glioma cell lines. Our results demonstrate that ZFX plays a crucial role in glioma proliferation and survival, confirming recent reports. We also show for the first time that ZFX knockdown decreases the in vivo growth potential of U87 glioma xenografts in both subcutaneous and intracranial models in nude mice. We conclude that lentivirus-mediated RNAi targeting of ZFX may serve as a promising strategy for glioma therapy.|
|Zhichuan Zhu; Kui Li; Dafeng Xu; Yongjie Liu; Hailiang Tang; Qing Xie; Liqian Xie; Jiwei Liu; Hongtao Wang; Ye Gong; Zelan Hu; Jing Zheng|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2013-01-16|
|Title: Journal of neuro-oncology Volume: 112 ISSN: 1573-7373 ISO Abbreviation: J. Neurooncol. Publication Date: 2013 Mar|
|Created Date: 2013-02-27 Completed Date: 2013-09-05 Revised Date: 2013-12-05|
Medline Journal Info:
|Nlm Unique ID: 8309335 Medline TA: J Neurooncol Country: United States|
|Languages: eng Pagination: 17-25 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Antiviral Agents / pharmacology
Brain Neoplasms / genetics*, pathology*
Cell Cycle / drug effects, genetics
Cell Line, Tumor
Cell Proliferation* / drug effects
Cell Survival / drug effects, genetics
Glioma / genetics*, pathology
In Situ Nick-End Labeling
Kruppel-Like Transcription Factors / genetics, metabolism*
Lentivirus / genetics, physiology
Mice, Inbred BALB C
Oncogene Protein v-akt / metabolism
RNA, Small Interfering / pharmacology
Signal Transduction / drug effects
Tumor Stem Cell Assay
Tunicamycin / pharmacology
Up-Regulation / drug effects, genetics
Xenograft Model Antitumor Assays
beta-Galactosidase / genetics, metabolism
|0/Antiviral Agents; 0/Kruppel-Like Transcription Factors; 0/RNA, Small Interfering; 0/zinc finger protein, X-linked; 11089-65-9/Tunicamycin; EC 184.108.40.206/Oncogene Protein v-akt; EC 220.127.116.11/beta-Galactosidase|
|J Neurooncol. 2013 Jul;113(3):533
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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