| ZBP-89 enhances Bak expression and causes apoptosis in hepatocellular carcinoma cells. | |
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MedLine Citation:
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PMID: 20850481 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ZBP-89 can enhance tumor cells to death stimuli. However, the molecular mechanism leading to the inhibitory effect of ZBP-89 is unknown. In this study, 4 liver cell lines were used to screen for the target of ZBP-89 on cell death pathway. The identified Bak was further analyzed for its role in ZBP-89-mediated apoptosis. The result showed that ZBP-89 significantly and time-dependently induced apoptosis. It significantly upregulated the level of pro-apoptotic Bak. ZBP-89 targeted a region between -457 and -407 of human Bak promoter to stimulate Bak expression based on the findings of Bak promoter luciferase report gene assay and electrophoretic mobility shift assay. ZBP-89-induced Bak increase and ZBP-89-mediated apoptosis were markedly suppressed by Bak siRNA, confirming that Bak was specifically targeted by ZBP-89 to facilitate apoptosis. In conclusion, this study demonstrated that ZBP-89 significantly induced apoptosis of HCC cells via promoting Bak level. |
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Authors:
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Ann K Y To; George G Chen; Ursula P F Chan; Caiguo Ye; Jing P Yun; Rocky L K Ho; Art Tessier; Juanita L Merchant; Paul B S Lai |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-17 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1813 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-03 Completed Date: 2011-03-22 Revised Date: 2011-03-24 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 222-30 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Blotting, Western Carcinoma, Hepatocellular / genetics, metabolism, pathology* Cell Proliferation DNA-Binding Proteins / genetics, metabolism* Electrophoretic Mobility Shift Assay Humans Liver Neoplasms / genetics, metabolism, pathology* Luciferases / metabolism Promoter Regions, Genetic / genetics RNA, Small Interfering / genetics Transcription Factors / genetics, metabolism* Tumor Cells, Cultured bcl-2 Homologous Antagonist-Killer Protein / antagonists & inhibitors, genetics*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK-55732/DK/NIDDK NIH HHS; R01 DK055732-08/DK/NIDDK NIH HHS; R01 DK055732-09/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/BAK1 protein, human; 0/DNA-Binding Proteins; 0/RNA, Small Interfering; 0/Transcription Factors; 0/ZNF148 protein, human; 0/bcl-2 Homologous Antagonist-Killer Protein; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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