Document Detail


Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
MedLine Citation:
PMID:  19349079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Newborn foals are very susceptible to infections by opportunistic pathogens such as Rhodococcus equi. This susceptibility is thought to be due to the immaturity of their immune system, in particular their inability to produce interferon-gamma. This deficiency may result from an insufficiency in accessory signals. We therefore compared monocyte-derived dendritic cells (MoDC) from foals and from adult horses. CD172, MHC-I and MHC-II were generally expressed on more than 90% MoDC from foals and adults. CD1w2(+)CD86(+) cells tended to be less represented in 2-3-week-old foals than in adults. This difference was significant among CD14(-) cells. The percentage of CD14(-)CD1w2(+)CD86(+) cells tended to be increased at 3 months. This suggests that very young foal dendritic cells are quantitatively less mature than their adult counterparts. The expression of IL-1, IL-12, IL-15 and IL-18 mRNA was not different in foal and adult MoDC, but the levels of TNF-alpha, IL-10, MCP-1 and TGF-beta were lower in foal cells. TNF-alpha and IL-10 expression was increased by LPS; TNF-alpha even reached the level of adult MoDC. This may mean that the lack of IFN-gamma in foals is not due to decreased levels of IL-12, IL-15 or IL-18, but rather to lower constitutive levels of TNF-alpha.
Authors:
Catherine Mérant; Cormac C Breathnach; Katharina Kohler; Cetewayo Rashid; Patricia Van Meter; David W Horohov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-03-14
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  131     ISSN:  1873-2534     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Antigens, CD1 / analysis
Antigens, CD14 / analysis
Antigens, CD86 / analysis
Cytokines / biosynthesis
Dendritic Cells / immunology*,  ultrastructure
Horses / immunology*
Lipopolysaccharides / immunology
Monocytes / cytology*
Phenotype
Chemical
Reg. No./Substance:
0/Antigens, CD1; 0/Antigens, CD14; 0/Antigens, CD86; 0/Cytokines; 0/Lipopolysaccharides

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