Document Detail


You deserve what you eat: lessons learned from the study of the melanin-concentrating hormone (MCH)-deficient mice.
MedLine Citation:
PMID:  20966023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Diet plays a crucial role in the development of obesity and insulin resistance via multiple mechanisms. Saturated fatty acids can directly trigger tissue specific proinflammatory pathways via Toll-like receptor-4 (TLR4)-dependent mechanisms. Moreover, diet can change the gut microbiome and increase gut permeability. However, very few studies have addressed the obesity-independent role of diet. Dissecting the effects of diet from those of obesity per se will enhance our understanding of the underlying pathogenesis, and, at the translational level, advance our treatment approaches for obesity and its co-morbidities.
METHODS: Melanin-concentrating hormone (MCH) is an important regulator of appetite and energy balance. MCH-deficient mice are resistant to diet-induced obesity, primarily due to increased locomotor activity. We took advantage of the unique phenotype of these mice to examine the metabolic and inflammatory consequences of a 15-week consumption of a diet high in saturated fat.
RESULTS: MCH-deficient mice chronically exposed to a high-fat diet gain less weight compared to their wild-type littermates, despite similar food intake, and are protected from hepatosteatosis. They also lack obesity-associated upregulation of serum leptin and insulin levels and have improved total body insulin sensitivity. Nevertheless, we found indistinguishable liver-specific innate immune responses in both genotypes associated with high-fat feeding, which involved activation of TLR4 and its downstream effectors, MyD88, p38 MAP kinase and STAT-3.
CONCLUSIONS: Our findings indicate that high-fat feeding is deleterious to the liver, independently of the obesity status. They also suggest that MCH is not necessary for the TLR4-dependent immune response triggered by the high-fat diet.
Authors:
Yan Wang; Dimitrios C Ziogas; Sudha Biddinger; Efi Kokkotou
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-21
Journal Detail:
Title:  Gut     Volume:  59     ISSN:  1468-3288     ISO Abbreviation:  Gut     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-12     Completed Date:  2010-12-10     Revised Date:  2011-07-26    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  England    
Other Details:
Languages:  eng     Pagination:  1625-34     Citation Subset:  AIM; IM    
Affiliation:
Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism
Adiposity / physiology
Animals
Blood Glucose / metabolism
Body Weight / physiology
Dietary Fats / administration & dosage*,  pharmacology
Eating / physiology
Gene Expression Regulation / drug effects
Gluconeogenesis / drug effects,  physiology
Glucose Tolerance Test
Hypothalamic Hormones / deficiency*,  physiology
Immunity, Innate
Insulin / blood
Leptin / blood
Lipid Metabolism / drug effects,  physiology
Lipogenesis / drug effects,  physiology
Liver / immunology,  metabolism
Male
Melanins / deficiency*,  physiology
Mice
Mice, Knockout
Pituitary Hormones / deficiency*,  physiology
Signal Transduction / physiology
Thinness / metabolism,  physiopathology
Toll-Like Receptor 4 / metabolism
Grant Support
ID/Acronym/Agency:
5 P30 CA06516/CA/NCI NIH HHS; R01 DK080058-03/DK/NIDDK NIH HHS; R01DK080058/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Fats; 0/Hypothalamic Hormones; 0/Leptin; 0/Melanins; 0/Pituitary Hormones; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 4; 11061-68-0/Insulin; 67382-96-1/melanin-concentrating hormone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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