Document Detail

YopK regulates the Yersinia pestis type III secretion system from within host cells.
MedLine Citation:
PMID:  21205017     Owner:  NLM     Status:  MEDLINE    
The pathogenic Yersinia species share a conserved type III secretion system, which delivers cytotoxic effectors known as Yops into target mammalian cells. In all three species, YopK (also called YopQ) plays an important role in regulating this process. In cell culture infections, yopK mutants inject higher levels of Yops, leading to increase cytotoxicity; however, in vivo the same mutants are highly attenuated. In this work, we investigate the mechanism behind this paradox. Using a β-lactamase reporter assay to directly measure the effect of YopK on translocation, we demonstrated that YopK controls the rate of Yop injection. Furthermore, we find that YopK cannot regulate effector Yop translocation from within the bacterial cytosol. YopE is also injected into host cells and was previously shown to contribute to regulation of the injectisome. In this work we show that YopK and YopE work at different steps to regulate Yop injection, with YopK functioning independently of YopE. Finally, by expressing YopK within tissue culture cells, we confirm that YopK regulates translocation from inside the host cell, and we show that cells pre-loaded with YopK are resistant to Yop injection. These results suggest a novel role for YopK in controlling the Yersinia type III secretion system.
Rebecca Dewoody; Peter M Merritt; Andrew S Houppert; Melanie M Marketon
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-19
Journal Detail:
Title:  Molecular microbiology     Volume:  79     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-10     Completed Date:  2011-06-20     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1445-61     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
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MeSH Terms
Bacterial Outer Membrane Proteins / genetics,  metabolism*
CHO Cells
Gene Expression Regulation, Bacterial
Host-Pathogen Interactions
Plague / microbiology*
Protein Transport
Yersinia pestis / genetics,  metabolism*
Grant Support
2-U54-AI-057153/AI/NIAID NIH HHS; R21 AI083660/AI/NIAID NIH HHS; R21 AI083660-01/AI/NIAID NIH HHS; R21 AI083660-02/AI/NIAID NIH HHS; R21AI083660/AI/NIAID NIH HHS; T32 GMOO7757//PHS HHS
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins

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