Document Detail


Yield and post-yield behavior of mussel byssal thread: a self-healing biomolecular material.
MedLine Citation:
PMID:  11710048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Byssal threads provide marine mussels with the tenacity to remain sessile in habitats of high flow. Under uniaxial tension, byssal threads are typical of other biological and synthetic fibers in exhibiting an initial linear region followed by yield. They differ, however, in their capacity to recover or "self-heal" following yield. We have examined the effect of urea, dithiothreitol (DTT), and metal-chelating agents such as ethylenediamine tetraacetic acid (EDTA) in perturbing the modulus, yield point, and energy dissipated in distal byssal threads stretched cyclically in seawater to a strain of 0.7. Threads stretched in the presence of 8 M urea or DTT show a complete abolition of yield point, while those washed clean of urea and DTT prior to stretching approach native controls. Threads stretched in the presence of EDTA show no effect; however, preincubation of distal threads in EDTA for 24 h results in a loss of yield point if stretched in metal-deficient seawater and normal behavior in natural seawater. The results indicate that while protein unfolding and disruption of disulfide linkages or chelate complexes compromises the yield strength of distal byssal fibers, there is typically a rapid recovery in natural sea water.
Authors:
E Vaccaro; J H Waite
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomacromolecules     Volume:  2     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:  2001  
Date Detail:
Created Date:  2001-11-16     Completed Date:  2002-01-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  906-11     Citation Subset:  IM    
Affiliation:
Marine Science Institute and Molecular, Cellular and Developmental Biology Department, University of California at Santa Barbara, Santa Barbara, California 93106, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopolymers / chemistry,  isolation & purification*
Bivalvia / chemistry*
Chelating Agents
Collagen / chemistry,  isolation & purification
Dithiothreitol
Edetic Acid
Macromolecular Substances
Protein Denaturation
Proteins / chemistry,  isolation & purification
Seawater
Urea
Grant Support
ID/Acronym/Agency:
5 R01 DE10042/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Biopolymers; 0/Chelating Agents; 0/Macromolecular Substances; 0/Proteins; 0/adhesive protein, mussel; 3483-12-3/Dithiothreitol; 57-13-6/Urea; 60-00-4/Edetic Acid; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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