Document Detail


YfdW and YfdU are required for oxalate-induced acid tolerance in Escherichia coli K-12.
MedLine Citation:
PMID:  23335415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Escherichia coli has several mechanisms for surviving low-pH stress. We report that oxalic acid, a small-chain organic acid (SCOA), induces a moderate acid tolerance response (ATR) in two ways. Adaptation of E. coli K-12 at pH 5.5 with 50 mM oxalate and inclusion of 25 mM oxalate in pH 3.0 minimal challenge medium separately conferred protection, with 67% ± 7% and 87% ± 17% survival after 2 h, respectively. The combination of oxalate adaptation and oxalate supplementation in the challenge medium resulted in increased survival over adaptation or oxalate in the challenge medium alone. The enzymes YfdW, a formyl coenzyme A (CoA) transferase, and YfdU, an oxalyl-CoA decarboxylase, are required for the adaptation effect but not during challenge. Unlike other SCOAs, this oxalate ATR is not a part of the RpoS regulon but appears to be linked to the signal protein GadE. We theorize that this oxalate ATR could enhance the pathogenesis of virulent E. coli consumed with oxalate-containing foods like spinach.
Authors:
Elise M Fontenot; Karen E Ezelle; Lauren N Gabreski; Eleanor R Giglio; John M McAfee; Alexandria C Mills; Maryam N Qureshi; Kristin M Salmon; Cory G Toyota
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-18
Journal Detail:
Title:  Journal of bacteriology     Volume:  195     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-12     Completed Date:  2013-05-03     Revised Date:  2014-05-16    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1446-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acids / toxicity*
Adaptation, Physiological
Carboxy-Lyases / genetics,  metabolism*
Coenzyme A-Transferases / genetics,  metabolism*
Colony Count, Microbial
Culture Media / chemistry
Escherichia coli K12 / drug effects*,  genetics*,  metabolism
Escherichia coli Proteins / genetics,  metabolism*
Hydrogen-Ion Concentration
Microbial Viability / drug effects
Oxalates / metabolism*
Grant Support
ID/Acronym/Agency:
P20 GM103476/GM/NIGMS NIH HHS; P20GM103476/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Acids; 0/Culture Media; 0/Escherichia coli Proteins; 0/Oxalates; EC 2.8.3.-/Coenzyme A-Transferases; EC 2.8.3.-/YfdW protein, E coli; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.8/oxalyl CoA decarboxylase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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