Document Detail

Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling.
MedLine Citation:
PMID:  22565313     Owner:  NLM     Status:  MEDLINE    
Intestinal barrier function requires intricate cooperation between intestinal epithelial cells and immune cells. Enteropathogens are able to invade the intestinal lymphoid tissue known as Peyer's patches (PPs) and disrupt the integrity of the intestinal barrier. However, the underlying molecular mechanisms of this process are poorly understood. In mice infected with Yersinia pseudotuberculosis, we found that PP barrier dysfunction is dependent on the Yersinia virulence plasmid and the expression of TLR-2 by hematopoietic cells, but not by intestinal epithelial cells. Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspase-1 and produced IL-1β. In turn, IL-1β increased NF-κB and myosin light chain kinase activation in intestinal epithelial cells, thus disrupting the intestinal barrier by opening the tight junctions. Therefore, Y. pseudotuberculosis subverts intestinal barrier function by altering the interplay between immune and epithelial cells during infection.
Camille Jung; Ulrich Meinzer; Nicolas Montcuquet; Elodie Thachil; Danielle Château; Raphaële Thiébaut; Maryline Roy; Ziad Alnabhani; Dominique Berrebi; Monique Dussaillant; Eric Pedruzzi; Sophie Thenet; Nadine Cerf-Bensussan; Jean-Pierre Hugot; Frederick Barreau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-08
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  122     ISSN:  1558-8238     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-01     Completed Date:  2012-08-20     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2239-51     Citation Subset:  AIM; IM    
Université Paris-Diderot, UMR843, Paris, France.
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MeSH Terms
Caco-2 Cells
Caspase 1 / genetics,  immunology
Enzyme Activation / genetics,  immunology
Hematopoietic Stem Cells / immunology*,  microbiology,  pathology
Interleukin-1beta / genetics,  immunology
Intestinal Mucosa / immunology*,  microbiology,  pathology
Mice, Knockout
Monocytes / immunology,  microbiology,  pathology
Myosin-Light-Chain Kinase / genetics,  immunology
NF-kappa B / genetics,  immunology
Peyer's Patches / immunology*,  microbiology,  pathology
Signal Transduction / genetics,  immunology*
Toll-Like Receptor 2 / genetics,  immunology*
Yersinia pseudotuberculosis / genetics,  immunology*
Yersinia pseudotuberculosis Infections / genetics,  immunology*,  pathology
Reg. No./Substance:
0/Interleukin-1beta; 0/NF-kappa B; 0/Tlr2 protein, mouse; 0/Toll-Like Receptor 2; EC Kinase; EC 1

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