| Yellow lupine cyclophilin interacts with nucleic acids. | |
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MedLine Citation:
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PMID: 18782068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate properties of yellow lupine cytosolic cyclophilin, an expression vector pET15CYP was constructed. The CyP cDNA (GenBank accession no.Y16088) reveals an open reading frame of 172 amino acids with the conserved tryptophan residue at position 128 and an insertion of seven amino acids spanning positions 48-54. Yellow lupine cyclophilin, purified after expression in E. coli cells, exhibits peptidyl-prolyl cis/trans isomerase activity when assayed with a synthetic oligopeptide. We have demonstrated that the recombinant cyclophilin is able to interact with nucleic acids, both single and double stranded DNA fragments as well as RNA. |
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Authors:
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Katarzyna Nuc; Krzysztof Leśniewicz; Przemysław Nuc; Ryszard Słomski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein and peptide letters Volume: 15 ISSN: 0929-8665 ISO Abbreviation: Protein Pept. Lett. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-09-10 Completed Date: 2008-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9441434 Medline TA: Protein Pept Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 719-23 Citation Subset: IM |
Affiliation:
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University of Natural Sciences, Department of Biochemistry and Biotechnology, ul. Wołyńska 35, 60-637 Poznań, Poland. ktnuc@owl.au.poznan.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cyclophilins / genetics, metabolism* DNA, Complementary / genetics DNA, Plant / genetics Escherichia coli / genetics Gene Expression Genes, Plant Genetic Vectors Kinetics Lupinus / enzymology*, genetics Nucleic Acids / metabolism* Recombinant Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA, Plant; 0/Nucleic Acids; 0/Recombinant Proteins; EC 5.2.1.-/Cyclophilins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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