| Yeast response and tolerance to polyamine toxicity involving the drug:H+ antiporter Qdr3 and the transcription factors Yap1 and Gcn4. | |
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MedLine Citation:
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PMID: 21148207 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The yeast QDR3 gene encodes a plasma membrane drug:H+ antiporter of the DHA1 family that was described as conferring resistance against drugs (quinidine, cisplatin, bleomycin) and the herbicide barban, similar to its close homolog QDR2. In this work, a new physiological role for Qdr3 in polyamine homeostasis is proposed. QDR3 is shown to confer resistance to polyamines (spermine and spermidine), but, unlike Qdr2, also a determinant of resistance to polyamines, Qdr3 has no apparent role in K+ homeostasis. QDR3 transcription is up-regulated in yeast cells exposed to spermine or spermidine in the dependency of the transcription factors Gcn4, which controls amino acid homeostasis, and Yap1, the main regulator of oxidative stress response. Yap1 was found to be a major determinant of polyamine stress resistance in yeast, being accumulated in the nucleus of yeast cells exposed to spermidine-induced stress. QDR3 transcript levels were also found to increase under nitrogen or amino acid limitation, this regulation being also dependent on Gcn4. Consistent with the concept that Qdr3 plays a role in polyamine homeostasis, QDR3 expression was found to decrease the intracellular accumulation of 3H-spermidine, playing a role in the maintenance of the plasma membrane potential in spermidine stressed cells. |
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Authors:
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Miguel Cacho Teixeira; Tânia Rodrigues Cabrito; Zaitunnissa M Hanif; Rita Criner Vargas; Sandra Tenreiro; Isabel Sá-Correia |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-9 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: - ISSN: 1465-2080 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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IBB - Institute for Biotechnology and Bioengineering, Instituto Superior T&eacnico, Lisboa, Portugal. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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