| Yeast cultures with UCP1 uncoupling activity as a heating device. | |
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MedLine Citation:
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PMID: 19761877 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Uncoupling proteins (UCPs) are mitochondrial transporters that facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24h through a thermocouple connected to a data logger. Under these conditions, significant temperature increases (1 degrees C) in the media were recorded when yeast strains expressing endogenously active UCP1 mutants were grown. This is the first direct evidence, in a eukaryotic microbial model, of a temperature rise associated with uncoupling activity, and could be seen as the first step toward developing a biological heating device. |
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Authors:
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Joaquina Delás; Meritxell Notari; Jaume Forés; Joaquín Pechuan; Manuel Porcar; Emilio Navarro; Arnau Montagud; Minerva Baguena; Juli Peretó; Pedro Fernández de Córdoba; M Mar González-Barroso; Eduardo Rial; Andrés Moya; Javier Urchueguía |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-15 |
Journal Detail:
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Title: New biotechnology Volume: 26 ISSN: 1876-4347 ISO Abbreviation: N Biotechnol Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 300-6 Citation Subset: IM |
Affiliation:
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Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Apartado Postal 22085, 46071 València, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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