Document Detail


Yeast cells can access distinct quiescent states.
MedLine Citation:
PMID:  21289062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We conducted a phenotypic, transcriptional, metabolic, and genetic analysis of quiescence in yeast induced by starvation of prototrophic cells for one of three essential nutrients (glucose, nitrogen, or phosphate) and compared those results with those obtained with cells growing slowly due to nutrient limitation. These studies address two related questions: (1) Is quiescence a state distinct from any attained during mitotic growth, and (2) does the nature of quiescence differ depending on the means by which it is induced? We found that either limitation or starvation for any of the three nutrients elicits all of the physiological properties associated with quiescence, such as enhanced cell wall integrity and resistance to heat shock and oxidative stress. Moreover, the starvations result in a common transcriptional program, which is in large part a direct extrapolation of the changes that occur during slow growth. In contrast, the metabolic changes that occur upon starvation and the genetic requirements for surviving starvation differ significantly depending on the nutrient for which the cell is starved. The genes needed by cells to survive starvation do not overlap the genes that are induced upon starvation. We conclude that cells do not access a unique and discrete G(0) state, but rather are programmed, when nutrients are scarce, to prepare for a range of possible future stressors. Moreover, these survival strategies are not unique to quiescence, but are engaged by the cell in proportion to nutrient scarcity.
Authors:
Maja M Klosinska; Christopher A Crutchfield; Patrick H Bradley; Joshua D Rabinowitz; James R Broach
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-02-02
Journal Detail:
Title:  Genes & development     Volume:  25     ISSN:  1549-5477     ISO Abbreviation:  Genes Dev.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-17     Completed Date:  2011-04-27     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  336-49     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / drug effects,  genetics,  physiology*
Cell Proliferation / drug effects
Cell Survival / drug effects,  genetics
Cluster Analysis
Gene Expression Profiling
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Fungal / drug effects
Genes, Developmental / drug effects,  physiology
Glucose / pharmacology
Metabolic Networks and Pathways / drug effects,  genetics
Models, Biological
Nitrogen / pharmacology
Organisms, Genetically Modified
Phosphates / pharmacology
Saccharomyces cerevisiae / genetics,  metabolism,  physiology
Starvation / genetics,  metabolism,  physiopathology
Transcription, Genetic / drug effects,  genetics
Yeasts / cytology,  genetics,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
GM076562/GM/NIGMS NIH HHS; P50 GM071508/GM/NIGMS NIH HHS; P50 GM071508/GM/NIGMS NIH HHS; R01 GM076562/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Phosphates; 50-99-7/Glucose; 7727-37-9/Nitrogen
Comments/Corrections

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