| Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets. | |
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MedLine Citation:
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PMID: 22393365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In Saccharomyces cerevisiae, the G1 cyclin/cyclin-dependent kinase (CDK) complexes Cln1,-2,-3/Cdk1 promote S phase entry during the mitotic cell cycle but do not function during meiosis. It has been proposed that the meiosis-specific protein kinase Ime2, which is required for normal timing of pre-meiotic DNA replication, is equivalent to Cln1,-2/Cdk1. These two CDK complexes directly catalyze phosphorylation of the B-type cyclin/CDK inhibitor Sic1 during the cell cycle to enable its destruction. As a result, Clb5,-6/Cdk1 become activated and facilitate initiation of DNA replication. While Ime2 is required for Sic1 destruction during meiosis, evidence now suggests that Ime2 does not directly catalyze Sic1 phosphorylation to target it for destabilization as Cln1,-2/Cdk1 do during the cell cycle. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrated that Sic1 is eventually degraded in meiotic cells lacking the IME2 gene (ime2Δ), supporting an indirect role of Ime2 in Sic1 destruction. We further examined global RNA expression comparing wild type and ime2Δ cells. Analysis of these expression data has provided evidence that Ime2 is required early in meiosis for normal transcription of many genes that are also periodically expressed during late G1 of the cell cycle. CONCLUSIONS/SIGNIFICANCE: Our results place Ime2 at a position in the early meiotic pathway that lies upstream of the position occupied by Cln1,-2/Cdk1 in the analogous cell cycle pathway. Thus, Ime2 may functionally resemble Cln3/Cdk1 in promoting S phase entry, or it could play a role even further upstream in the corresponding meiotic cascade. |
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Authors:
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George S Brush; Nicole A Najor; Alan A Dombkowski; Daniela Cukovic; Kara E Sawarynski |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-02-29 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-03-06 Completed Date: 2012-07-23 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e31575 Citation Subset: IM |
Affiliation:
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Department of Oncology, Wayne State University School of Medicine, Detroit, Michigan, United States of America. brushg@karmanos.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs CDC2 Protein Kinase / metabolism Catalysis Cell Cycle Cyclin-Dependent Kinase Inhibitor Proteins / metabolism Cyclin-Dependent Kinases / metabolism* Cyclins / metabolism DNA Replication Epistasis, Genetic Gene Expression Profiling Intracellular Signaling Peptides and Proteins / genetics, physiology* Meiosis Models, Biological Models, Genetic Oligonucleotide Array Sequence Analysis Phosphorylation Ploidies Protein-Serine-Threonine Kinases / genetics, physiology* Repressor Proteins / metabolism Saccharomyces cerevisiae / metabolism*, physiology Saccharomyces cerevisiae Proteins / genetics, metabolism, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P30CA22453/CA/NCI NIH HHS; T32CA009531/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CLN1 protein, S cerevisiae; 0/CLN2 protein, S cerevisiae; 0/Cyclin-Dependent Kinase Inhibitor Proteins; 0/Cyclins; 0/Intracellular Signaling Peptides and Proteins; 0/Repressor Proteins; 0/SIC1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Whi5 protein, S cerevisiae; EC 2.7.11.1/IME2 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/Cyclin-Dependent Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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