Document Detail

Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging.
MedLine Citation:
PMID:  16436509     Owner:  NLM     Status:  MEDLINE    
Apoptosis-inducing factor (AIF) and AIF-homologous mitochondrion-associated inducer of death (AMID) are both mitochondrial flavoproteins that trigger caspase-independent apoptosis. Phylogenetic analysis suggests that these two proteins evolutionarily diverge back from their common prokaryote ancestor. Compared with AIF, the proapoptotic nature of AMID and its mode of action are much less clarified. Here, we show that overexpression of yeast AMID homologue internal NADH dehydrogenase (NDI1), but not external NADH dehydrogenase (NDE1), can cause apoptosis-like cell death, and this effect can be repressed by increased respiration on glucose-limited media. This result indicates that the regulatory network of energy metabolism, in particular the cross-talk between mitochondria and the rest of the cell, is involved in Ndi1p-induced yeast cell apoptosis. The apoptotic effect of NDI1 overexpression is associated with increased production of reactive oxygen species (ROS) in mitochondria. In addition, NDI1 overexpression in sod2 background causes cell lethality in both fermentable and semifermentable media. Interruption of certain components in the electron transport chain can suppress the growth inhibition from Ndi1p overexpression. We finally show that disruption of NDI1 or NDE1 decreases ROS production and elongates the chronological life span of yeast, accompanied by the loss of survival fitness. Implication of these findings for Ndi1p-induced apoptosis is discussed.
Wei Li; Libo Sun; Qiuli Liang; Juan Wang; Weike Mo; Bing Zhou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-25
Journal Detail:
Title:  Molecular biology of the cell     Volume:  17     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-29     Completed Date:  2006-06-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1802-11     Citation Subset:  IM    
State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
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MeSH Terms
Electron Transport
Electron Transport Complex I
Gene Deletion
Mitochondria / metabolism*
NADH Dehydrogenase / classification,  genetics,  metabolism*
Reactive Oxygen Species / metabolism*
Saccharomyces cerevisiae / enzymology,  growth & development*,  ultrastructure
Saccharomyces cerevisiae Proteins / classification,  genetics,  metabolism*
Superoxide Dismutase / genetics
Time Factors
Transcriptional Activation
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Saccharomyces cerevisiae Proteins; EC Dismutase; EC dismutase 2; EC Transport Complex I; EC protein, S cerevisiae; EC Dehydrogenase

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