Document Detail


Yak lactate dehydgogenase A4: purification, properties, and cDNA cloning.
MedLine Citation:
PMID:  18776664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactate dehydrogenase A4 (LDH-A4) was purified for yak skeletal muscle. Michaelis constant (Km) analysis showed that yak LDH-A4 for pyruvate was significantly higher than that of cattle. cDNA cloning of LDH-A revealed two amino acid substitutions between yak and cattle. We suggest that the higher Km of yak LDH-A4 might be a result of molecular adaptation to a hypoxic environment.
Authors:
Yucai Zheng; Xiaohui Si; Qinghua He; Suyu Jin; Jian Hong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  72     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-24     Completed Date:  2008-12-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2448-51     Citation Subset:  IM    
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, PR China. yucaizheng01@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acid Substitution
Animals
Cattle / genetics*
Cloning, Molecular
DNA, Complementary / genetics,  isolation & purification
Isoenzymes / genetics*,  metabolism*
Kinetics
L-Lactate Dehydrogenase / genetics*,  isolation & purification*,  metabolism
Lactic Acid / isolation & purification
Models, Molecular
Molecular Sequence Data
Muscle, Skeletal / enzymology
Pyruvic Acid / isolation & purification
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Isoenzymes; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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