Document Detail


YKL-40 Induces IL-8 Expression from Bronchial Epithelium via MAPK (JNK and ERK) and NF-κB Pathways, Causing Bronchial Smooth Muscle Proliferation and Migration.
MedLine Citation:
PMID:  23197259     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Recently, the serum levels of YKL-40, a chitinase-like glycoprotein, have been shown to be significantly elevated in asthmatics and are associated with asthma severity. Although these studies raise the possibility that YKL-40 may influence asthma, the mechanisms remain unknown. This study firstly investigated the mechanisms involved in YKL-40-mediated inflammation in human bronchial epithelial cells (HBECs) and analyzed the soluble factors secreted by bronchial epithelial cells exposed to YKL-40 that were responsible for increasing proliferation and migration of primary normal human bronchial smooth muscle cells (BSMCs). YKL-40-induced inflammation was assayed in two HBECs (BEAS-2B cell line and primary HBECs). In addition, we treated BEAS-2B cells and HBECs with YKL-40 and added the conditioned culture media to BSMCs. The proliferation and migration of BSMCs were determined by premixed WST-1 cell proliferation reagent (Clontech Laboratories) and QCM chemotaxis migration assay (Millipore), respectively. Bronchial epithelial cells treated with YKL-40 resulted in a significant increase of IL-8 production, which was dependent on MAPK (JNK and ERK) and NF-κB pathways activation. YKL-40-induced IL-8 was found to further stimulate proliferation and migration of BSMCs, and the effects were inhibited after neutralizing IL-8. Through investigating the interaction of airway epithelium and smooth muscle, our findings implicate that YKL-40 may be involved in the inflammation of asthma by induction of IL-8 from epithelium, subsequently contributing to BSMC proliferation and migration. Moreover, inhibition of IL-8 signaling is a potential therapeutic target for YKL-40-induced inflammation and remodeling of asthma.
Authors:
Hao Tang; Yu Sun; Zhaoquan Shi; Hai Huang; Zheng Fang; Jiquan Chen; Qingyu Xiu; Bing Li
Related Documents :
15283759 - Current mechanisms of macula densa cell signalling.
15253109 - Dual effects of endothelin-1 (1-31): induction of mesangial cell migration and facilita...
7589989 - Stimulation of matrix metalloproteinase-dependent migration of t cells by eicosanoids.
7537959 - Proteoglycan production by human glomerular visceral epithelial cells and mesangial cel...
2254329 - Cadmium uptake by cells of renal origin.
3141399 - Mass quantitation of agonist-induced arachidonate release and icosanoid production in a...
9824499 - Immunosuppressant deoxyspergualin induces apoptotic cell death in dividing cells.
9563679 - Use of a chemically defined system for the direct comparison of inner cell mass and tro...
8060589 - Effects of pre-conditioning and microbial composition on the sensing efficacy of a bod ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  -     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Respiratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  SLAT regulates CD8+ T cell clonal expansion in a Cdc42- and NFAT1-dependent manner.
Next Document:  Lipoproteins are major targets of the polyclonal human T cell response to Mycobacterium tuberculosis...