| YIA6 Effects of GLP-1 eluting stem cell therapy on collagen remodelling, infarct size and apoptosis in a porcine model of myocardial infarction. | |
| | |
MedLine Citation:
|
PMID: 20801784 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Glucagon-like peptide-1 (GLP-1) is a gut incretin hormone with cardioprotective effects. Human stem cells secreting a GLP-1 fusion protein and encapsulated in an alginate matrix (GLP-1 CellBeads) have been developed as a novel therapeutic agent. This study investigated the effects of GLP-1 CellBeads on post-myocardial infarction (MI) healing in a porcine model. GLP-1 CellBeads were delivered to the left anterior descending coronary artery to create micro-infarcts, with cell-free beads as controls. Hearts were explanted at 1 and 4 weeks post-MI. Gross infarct size was measured as a percentage of left ventricular area. Tissue was analysed for inflammation (number of MAC 387 positive cells/mm(2)), apoptosis (% TUNEL positive cells) and collagen (% picrosirius red staining). Compared with controls (n=4), the GLP-1-treated group (n=6) exhibited less infarct at one (6.21%+/-0.64 vs 9.78%+/-1.80% 2LV, p=NS) and 4 weeks post-MI (4.7%+/-2.1 vs 21.8%+/-4.8% LV, p=0.02). Within the infarct there was increased inflammation in GLP-1-treated groups at both time points (1 week: 97.22%+/-19.62 vs 36.67%+/-7.78, p=0.01; 4 weeks: 24.2%+/-4.57 vs 12.3%+/-3.16, p=0.03). At 1 week, apoptosis rates in the infarct area were similar in both groups (1.46 %+/-0.57 vs 1.47 %+/-0.17, p=NS), with less apoptosis in the GLP-1-treated group at 4 weeks (0.51%+/-0.18 vs 1.84%+/-0.62, p=NS). Collagen content at 1 week was lower (5.14%+/-1.19 vs 9.95%+/-1.42, p=0.05) and at 4 weeks higher in the treated group (20.89%+/-8.25 vs 6.87%+/-2.92, p=NS). GLP-1 CellBeads have an effect on post-MI infarct size, inflammation and ventricular remodelling. These findings require further validation before clinical translation. |
| | |
Authors:
|
E J Wright; K A Farrell; C Wallrapp; P Geigle; A L Lewis; P W Stratford; N Malik; C M Holt |
Related Documents
:
|
19797174 - Induction of the cxc chemokine interferon-gamma-inducible protein 10 regulates the repa... 14550304 - Combination of angiopoietin-1 and vascular endothelial growth factor gene therapy enhan... 16418824 - Effects of g-csf on left ventricular remodeling and heart failure after acute myocardia... 233324 - Role of cyclic amp in myocardial cell function. 12407614 - Postoperative oral amiodarone as prophylaxis against atrial fibrillation after coronary... 7586254 - Development and characterization of a rapid assay for bedside determinations of cardiac... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Heart (British Cardiac Society) Volume: 96 ISSN: 1468-201X ISO Abbreviation: Heart Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9602087 Medline TA: Heart Country: England |
Other Details:
|
Languages: eng Pagination: e10-1 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro activity of ceftazidime+NXL104 against Pseudomonas aeruginosa and other non-fermenters.
Next Document: YIA5 PKC{delta} deficiency accelerates neointimal lesions in a mouse model of vascular injury involv...