Document Detail


Y-chromosome-specific fluorescence (f-body) of poorly decondensed bovine spermatozoa.
MedLine Citation:
PMID:  1382393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An account is given in this paper of a method of identifying the fluorescence body (f-body) as a marker of the Y-chromosome. Also covered by this method is poor decondensation of spermatozoal nuclei when exposed to action of 1.25% of papain, 0.155% of DTE, and 0.025% of DMSO. Quinacrine mustard was used as fluorescent stain, its final concentration being 0.0025%. For staining, spermatozoa were suspended for 1-5 h. Average f-body frequency accounted for 41.0 +/- 5.1% in 35 ejaculates from 22 bulls. The overall variation coefficient amounted to 12.4% and thus was higher than each of the single values individually recorded from six bulls which were involved with three or four ejaculates (3.6-7.0%). F-bodies could not be detected by the method generally used on human spermatozoa. The applicability of the f-body test to quantification of Y spermatozoa in experimental separation of androspermatozoa and gynospermatozoa is discussed and is demonstrated by an example.
Authors:
S Blottner; C Pitra; U Berger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Andrologia     Volume:  24     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:    1992 Sep-Oct
Date Detail:
Created Date:  1992-10-16     Completed Date:  1992-10-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  255-9     Citation Subset:  IM    
Affiliation:
Institute of Wild and Zoo Animal Research Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Nucleus / ultrastructure
Centrifugation, Density Gradient
Dimethyl Sulfoxide / pharmacology
Fluorescence
Male
Papain / pharmacology
Quinacrine Mustard
Spermatozoa / ultrastructure*
Staining and Labeling
Y Chromosome / ultrastructure*
Chemical
Reg. No./Substance:
4213-45-0/Quinacrine Mustard; 67-68-5/Dimethyl Sulfoxide; EC 3.4.22.2/Papain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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