Document Detail


Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.
MedLine Citation:
PMID:  25358887     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Aim. To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler® kit. Consensus DNA profiles were generated from 5 replicate experiments. Results. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.
Authors:
Lei Feng; Cheng Xu; Xianhai Zeng; Huaicai Zhang; Fan Yang; Wanshui Li; Zheng Tu; Caixia Li; Xxx Xy Yyy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Croatian medical journal     Volume:  55     ISSN:  1332-8166     ISO Abbreviation:  Croat. Med. J.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9424324     Medline TA:  Croat Med J     Country:  Croatia    
Other Details:
Languages:  eng     Pagination:  537-41     Citation Subset:  IM    
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