Document Detail

Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.
MedLine Citation:
PMID:  25358887     Owner:  NLM     Status:  In-Data-Review    
Aim. To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler® kit. Consensus DNA profiles were generated from 5 replicate experiments. Results. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.
Lei Feng; Cheng Xu; Xianhai Zeng; Huaicai Zhang; Fan Yang; Wanshui Li; Zheng Tu; Caixia Li; Xxx Xy Yyy
Related Documents :
3793797 - The cell cycle of symbiotic chlorella. iv. dna content of algae slowly increases during...
12374617 - Hydrogen peroxide inhibits cell cycle progression by inhibition of the spreading of mit...
16218827 - Cell cycle analysis of cultured porcine mammary cells.
3871457 - The roles of t cell factors in activation, cell cycle progression, and differentiation ...
17013867 - Three-dimensional collagen fiber remodeling by mesenchymal stem cells requires the inte...
15566957 - Hyperphosphorylation of extracellular regulated kinase 2 (erk2) and inhibition of jnk2 ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Croatian medical journal     Volume:  55     ISSN:  1332-8166     ISO Abbreviation:  Croat. Med. J.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9424324     Medline TA:  Croat Med J     Country:  Croatia    
Other Details:
Languages:  eng     Pagination:  537-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Genetic analysis of haplotype data for 23 Y-chromosome short tandem repeat loci in the Turkish popul...
Next Document:  Prevalence of urinary incontinence and its association with multimorbidity in women aged 50 years or...