| Xylanases of anaerobic fungus Anaeromyces mucronatus. | |
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MedLine Citation:
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PMID: 20680572 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The anaerobic fungus Anaeromyces mucronatus KF8 grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage saccharides including cellulase, endoglucanase, xylanase, alpha-xylosidase, beta-xylosidase, alpha-glucosidase, beta-glucosidase, beta-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extracellular fractions, but production into the medium was prevalent with the exception of intracellular beta-xylosidase, chitinases, N-acetylglucosaminidase, and lipase. Xylanase activity was predominant among the polysaccharide hydrolases. Extracellular production of xylanase was stimulated by the presence of cellobiose and oat spelt xylan. Zymogram of xylanases of strain KF8 grown on different carbon sources revealed several isoforms of xylanases with approximate molar masses ranging from 26 to 130 kDa. |
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Authors:
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Z Novotná; J Procházka; J Simůnek; K Fliegerová |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-03 |
Journal Detail:
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Title: Folia microbiologica Volume: 55 ISSN: 1874-9356 ISO Abbreviation: Folia Microbiol. (Praha) Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376757 Medline TA: Folia Microbiol (Praha) Country: United States |
Other Details:
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Languages: eng Pagination: 363-7 Citation Subset: IM |
Affiliation:
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Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic, 142 20, Prague 4, Czech Republic. novotna@iapg.cas.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Cellobiose / metabolism Cellulose / metabolism* Culture Media / chemistry Fungal Proteins / biosynthesis* Glycoside Hydrolases / biosynthesis*, chemistry, classification Molecular Weight Neocallimastigales / enzymology*, physiology Xylans / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fungal Proteins; 0/Xylans; 16462-44-5/Cellobiose; 9004-34-6/Cellulose; EC 3.2.1.-/Glycoside Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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