Document Detail


Xf phage invading the host cells with their protein coats.
MedLine Citation:
PMID:  1018174     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Xf phage coat protein associated with infected cells could not be removed by washing with antiserum and tris-EDTA buffer. Although the infected cells were consecutively washed 6 times with tris-EDTA buffer, the ratios of parental phage 6H-DNA to 14C-protein were not changed. A considerable amount of the parental 14C-protein and 3H-DNA in the original ratio were detected in the membrane and the soluble cytoplasmic fractions of infected cells. The studies of the change in Xf 14C-protein and 3H-DNA incorporation into the host cells and their release showed that DNA and protein penetrate together into the host cells during the first 60 min after infection (p.i.). While virtually all parental DNA was conserved, re-utilized and released from the infected cell 60 min p.i., no apparent release of parental protein was observed. Approx. 40% of the parental protein became degraded and could be washed from the infected cell after 90 min; the rest of the parental protein remained and probably was re-utilized by the host.
Authors:
T T Kuo; Y T Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general virology     Volume:  32     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1977-04-25     Completed Date:  1977-04-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  241-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / growth & development*,  metabolism
Cytoplasm / metabolism
DNA, Viral / metabolism
Spheroplasts / metabolism
Viral Proteins* / metabolism
Xanthomonas* / metabolism
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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