Document Detail


Xer-mediated site-specific recombination in vitro.
MedLine Citation:
PMID:  8605888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Xer site-specific recombination system acts at ColE1 cer and pSC101 psi sites to ensure that these plasmids are in a monomeric state prior to cell division. We show that four proteins, ArgR, PepA, XerC and XerD are necessary and sufficient for recombination between directly repeated cer sites on a supercoiled plasmid in vitro. Only PepA, XerC and XerD are required for recombination at psi in vitro. Recombination at cer and psi in vitro requires negative supercoiling and is exclusively intramolecular. Strand exchange at cer produces Holliday junction-containing products in which only the top strands have been exchanged. This reaction requires the catalytic tyrosine residue of Xer C but not that of XerD. Recombination at psi gives catenated circular resolution products. Strand exchange at psi is sequential. XerC catalyses the first (top) strand exchange to make a Holiday junction intermediate and XerD catalyses the second (bottom) strand exchange.
Authors:
S D Colloms; R McCulloch; K Grant; L Neilson; D J Sherratt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  15     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-05-17     Completed Date:  1996-05-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1172-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Oxford, UK.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites / genetics
DNA Nucleotidyltransferases / metabolism*
DNA, Superhelical / chemistry,  genetics,  metabolism
Escherichia coli / genetics*,  metabolism*
Escherichia coli Proteins*
Integrases*
Molecular Sequence Data
Nucleic Acid Conformation
Plasmids / chemistry,  genetics,  metabolism
Recombinases
Recombination, Genetic*
Substrate Specificity
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/DNA, Superhelical; 0/Escherichia coli Proteins; 0/Recombinases; 0/XerC protein, E coli; EC 2.7.7.-/DNA Nucleotidyltransferases; EC 2.7.7.-/Integrases; EC 2.7.7.-/XerD protein, E coli; EC 2.7.7.-/integron integrase IntI1
Comments/Corrections

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