Document Detail


Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation.
MedLine Citation:
PMID:  20172867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Grafting of testicular tissue into immunodeficient mice has been used to differentiate the neonatal testes from different animal species up to the level of complete spermatogenesis; however, this approach has not been successful for human testicular tissue. The aim of this study was to evaluate the capacity for differentiation of infant human testicular tissue grafts. METHODS AND RESULTS: Testicular tissue from a 3-month-old patient with testicular cancer was grafted into immunodeficient nude mice. At the time of grafting, A spermatogonia were the only germ cells present in the testicular tissue. B spermatogonia and first spermatocytes were observed at 7 months and 1 year after grafting, respectively. Positive immunostaining with antibodies against BOULE and CDC25A suggested that spermatocytes in the graft were not arrested but in meiosis. Furthermore, ultrastructural and immunohistochemical analyses showed that the onset of both Sertoli cell maturation and partial differentiation of Leydig cells preceded the appearance of spermatocytes. Differentiation of testicular cells was accelerated compared with in vivo development. CONCLUSIONS: Spermatogenesis in the xenograft of infant human testicular tissues proceeded successfully from the stage of spermatogonial stem cells until pachytene spermatocyte formation. The differentiation of Sertoli cells and Leydig cells was reproduced in a manner similar to that in normal testicular development. Grafting of infant human testicular tissue may be a powerful tool to examine the early period of human spermatogenesis and may pave the way for fertility preservation among infant patients.
Authors:
Y Sato; S Nozawa; M Yoshiike; M Arai; C Sasaki; T Iwamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-19
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  25     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1113-22     Citation Subset:  IM    
Affiliation:
Department of Urology, St. Marianna University School of Medicine, Kawasaki, Japan. ysato@nagasaki-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Fertility
Hemangioma / pathology,  therapy
Humans
Immunohistochemistry
Infant
Leydig Cells / cytology
Male
Mice
Mice, Nude
Microscopy, Electron, Transmission
RNA-Binding Proteins / metabolism
Sertoli Cells / cytology
Spermatocytes / cytology
Spermatogenesis
Spermatogonia / cytology
Testicular Neoplasms / pathology,  therapy
Testis / cytology,  growth & development*,  metabolism,  transplantation*
Transplantation, Heterologous
cdc25 Phosphatases / metabolism
Chemical
Reg. No./Substance:
0/BOLL protein, human; 0/RNA-Binding Proteins; EC 3.1.3.48/CDC25A protein, human; EC 3.1.3.48/cdc25 Phosphatases

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