| Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress. | |
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MedLine Citation:
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PMID: 19062051 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xanthone production in Hypericum perforatum (HP) suspension cultures in response to elicitation by Agrobacterium tumefaciens co-cultivation has been studied. RNA blot analyses of HP cells co-cultivated with A. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (PAL, phenylalanine ammonia lyase and 4CL, 4-coumarate:CoA ligase) and xanthone biosynthesis (BPS, benzophenone synthase). Analyses of HPLC chromatograms of methanolic extracts of control and elicited cells (HP cells that were co-cultivated for 24h with A. tumefaciens) have revealed a 12-fold increase in total xanthone concentration and also the emergence of many xanthones after elicitation. Methanolic extract of elicited cells exhibited significantly higher antioxidant and antimicrobial competence than the equivalent extract of control HP cells indicating that these properties have been significantly increased in HP cells after elicitation. Four major de novo synthesized xanthones have been identified as 1,3,6,7-tetrahydroxy-8-prenyl xanthone, 1,3,6,7-tetrahydroxy-2-prenyl xanthone, 1,3,7-trihydroxy-6-methoxy-8-prenyl xanthone and paxanthone. Antioxidant and antimicrobial characterization of these de novo xanthones have revealed that xanthones play dual function in plant cells during biotic stress: (1) as antioxidants to protect the cells from oxidative damage and (2) as phytoalexins to impair the pathogen growth. |
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Authors:
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Gregory Franklin; Luis F R Conceição; Erich Kombrink; Alberto C P Dias |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-04 |
Journal Detail:
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Title: Phytochemistry Volume: 70 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-02-09 Completed Date: 2009-05-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 60-8 Citation Subset: IM |
Affiliation:
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Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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metabolism* Antioxidants / metabolism* Hypericum / cytology*, metabolism* Molecular Structure Oxidative Stress Reactive Oxygen Species Rhizobium radiobacter / drug effects Xanthones / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Antioxidants; 0/Reactive Oxygen Species; 0/Xanthones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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