| Xanthohumol induces apoptosis in cultured 40-16 human colon cancer cells by activation of the death receptor- and mitochondrial pathway. | |
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MedLine Citation:
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PMID: 15995977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xanthohumol (XN) is one of the major prenylflavonoids found in hop cones (Humulus lupulus L.). In this study, we investigated the cell growth inhibitory potential of XN on cultured human colon cancer cells. Cell proliferation was measured by sulforhodamine B staining. Poly(ADP-ribose)polymerase (PARP) cleavage, activation of caspases-3, -7, -8, and -9, and Bcl-2 family protein expression were detected by Western blot analyses. XN significantly reduced proliferation of the HCT 116-derived colon cancer cell line 40--16. Half-maximal inhibitory concentrations decreased from 4.1 microM after 24 h treatment to 3.6 and 2.6 microM after 48 and 72 h incubation, respectively. Treatment with 15 microM XN for 48 h and with 5 microM for 72 h led to the detection of the cleaved 89 kDa fragment of 116 kDa PARP as an indication of apoptosis induction. Concomitantly, we observed activation and cleavage of the effector caspases-3 and -7, induced by activation of the initiator caspases -8 and -9. Expression of anti-apoptotic Bcl-2 was down regulated when the cells were treated with XN for 48--72 h. We conclude that induction of apoptosis by downregulation of Bcl-2 and activation of the caspase cascade may contribute to the chemopreventive or therapeutic potential of XN. |
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Authors:
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Lydia Pan; Hans Becker; Clarissa Gerhäuser |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 49 ISSN: 1613-4125 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-09-19 Completed Date: 2005-11-22 Revised Date: 2007-03-22 |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 837-43 Citation Subset: IM |
Affiliation:
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Division of Toxicology and Cancer Risk Factors, German Cancer Research Center, Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 Caspase 7 Caspase 8 Caspase 9 Caspases / metabolism* Cell Division / drug effects Cell Line, Tumor Colonic Neoplasms / pathology* Enzyme Activation Humans Humulus / chemistry Mitochondria / drug effects*, metabolism Poly(ADP-ribose) Polymerases / metabolism Propiophenones / pharmacology* Proto-Oncogene Proteins c-bcl-2 / analysis |
| Chemical | |
Reg. No./Substance:
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0/Propiophenones; 0/Proto-Oncogene Proteins c-bcl-2; 6754-58-1/xanthohumol; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP7 protein, human; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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