Document Detail

XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in Arabidopsis.
MedLine Citation:
PMID:  23204407     Owner:  NLM     Status:  MEDLINE    
Xyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH:XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of (27)Al-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.
Xiao Fang Zhu; Yuan Zhi Shi; Gui Jie Lei; Stephen C Fry; Bao Cai Zhang; Yi Hua Zhou; Janet Braam; Tao Jiang; Xiao Yan Xu; Chuan Zao Mao; Yuan Jiang Pan; Jian Li Yang; Ping Wu; Shao Jian Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-30
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-08-21     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4731-47     Citation Subset:  IM    
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
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MeSH Terms
Aluminum / toxicity*
Amino Acid Sequence
Arabidopsis / chemistry,  drug effects,  enzymology*,  genetics
Arabidopsis Proteins / genetics,  metabolism*
Cell Wall / metabolism
Chelating Agents / analysis,  metabolism
Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Plant
Glucans / analysis,  metabolism*
Glycosyltransferases / genetics,  metabolism
Mutagenesis, Insertional
Organ Specificity
Plant Leaves / chemistry,  drug effects,  enzymology,  genetics
Plant Roots / chemistry,  drug effects,  enzymology,  genetics
Plants, Genetically Modified
Polysaccharides / analysis,  metabolism
Recombinant Fusion Proteins
Seedling / chemistry,  drug effects,  enzymology,  genetics
Sequence Analysis, DNA
Xylans / analysis,  metabolism*
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Chelating Agents; 0/Glucans; 0/Polysaccharides; 0/Recombinant Fusion Proteins; 0/Xylans; 37294-28-3/xyloglucan; 7429-90-5/Aluminum; 8024-50-8/hemicellulose; EC 2.4.-/Glycosyltransferases; EC - xyloglucosyltransferase

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