| XRCC4 in G1 suppresses homologous recombination in S/G2, in G1 checkpoint-defective cells. | |
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MedLine Citation:
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PMID: 17057732 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Non-homologous end joining (NHEJ) and homologous recombination (HR) are two pathways that can compete or cooperate for DNA double-strand break (DSB) repair. NHEJ was previously shown to act throughout the cell cycle whereas HR is restricted to late S/G2. Paradoxically, we show here that defect in XRCC4 (NHEJ) leads to over-stimulation of HR when cells were irradiated in G1, not in G2. However, XRCC4 defect did not modify the strict cell cycle regulation for HR (i.e. in S/G2) as attested by (i) the formation of Rad51 foci in late S/G2 whatever the XRCC4 status, and (ii) the fact that neither Rad51 foci nor HR (gene conversion plus single-strand annealing) events induced by ionizing radiation were detected when cells were maintained blocked in G1. Finally, both gamma-H2AX analysis and pulse field gel electrophoresis showed that following irradiation in G1, some DSBs reached S/G2 in NHEJ-defective cells. Taken together, our results show that when cells are defective in G1/S arrest, DSB produced in G1 and left unrepaired by XRCC4 can be processed by HR but in late S/G2. |
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Authors:
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Y Saintigny; F Delacôte; D Boucher; D Averbeck; B S Lopez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-23 |
Journal Detail:
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Title: Oncogene Volume: 26 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-26 Completed Date: 2007-05-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 2769-80 Citation Subset: IM |
Affiliation:
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UMR CNRS 217, CEA, Fontenay aux Roses Cédex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured / radiation effects DNA Breaks, Double-Stranded* DNA-Binding Proteins / genetics, physiology* G1 Phase / genetics*, radiation effects G2 Phase / genetics*, radiation effects Gene Targeting Infrared Rays Mice Mice, Knockout Rad51 Recombinase / metabolism Recombination, Genetic* S Phase / genetics*, radiation effects |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/XRCC4 protein, mouse; EC 2.7.7.-/Rad51 Recombinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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