| XPteg (Xenopus proximal tubules-expressed gene) is essential for pronephric mesoderm specification and tubulogenesis. | |
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MedLine Citation:
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PMID: 19909807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retinoic acid (RA) signaling is important for the early steps of nephrogenic cell fate specification. Here, we report a novel target gene of RA signaling named XPteg (Xenopus proximal tubules-expressed gene) which is critical for pronephric development. XPteg starts to be expressed at the earliest stage of embryonic kidney specification and was restricted to the pronephric proximal tubules during kidney development. Anti-sense morpholino (MO)-mediated knockdown of XPteg perturbed formation of pronephros as demonstrated by reduced expression of pronephric tubule markers. Conversely, overexpression of XPteg promoted endogenous and ectopic expression of those markers and expanded pronephric tubules. Treatment of retinoic acid induced the expression of XPteg in the pronephric field without protein synthesis. Furthermore, we found that the pronephric defects caused by a dominant negative RA receptor could be rescued by coexpression of XPteg. Taken together, these results suggest that XPteg functions as a direct transcriptional target of RA signaling to regulate pronephric tubulogenesis in Xenopus early development. |
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Authors:
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Seung Joon Lee; Sanghee Kim; Sun-Cheol Choi; Jin-Kwan Han |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-10 |
Journal Detail:
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Title: Mechanisms of development Volume: 127 ISSN: 1872-6356 ISO Abbreviation: Mech. Dev. Publication Date: 2010 Jan-Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9101218 Medline TA: Mech Dev Country: Ireland |
Other Details:
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Languages: eng Pagination: 49-61 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Gene Expression Regulation, Developmental Homeodomain Proteins / metabolism In Situ Hybridization Intracellular Signaling Peptides and Proteins / metabolism, physiology* Kidney Tubules / embryology* Mesoderm / metabolism* Molecular Sequence Data Nephrons / embryology* Oligonucleotides, Antisense / genetics Paired Box Transcription Factors / metabolism Protein Structure, Tertiary Sequence Homology, Amino Acid Tretinoin / metabolism Xenopus Proteins / metabolism, physiology* Xenopus laevis / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Lim-1 protein, Xenopus; 0/Oligonucleotides, Antisense; 0/Paired Box Transcription Factors; 0/Pax8 protein, Xenopus; 0/Pteg protein, Xenopus; 0/Xenopus Proteins; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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