| XCL-2 is a novel m-type calpain and disrupts morphogenetic movements during embryogenesis in Xenopus laevis. | |
| | |
MedLine Citation:
|
PMID: 11576173 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We identified a novel cDNA, XCL-2, encoding an m-type calpain, a calcium-dependent intracellular protease. This protein has all characteristic structures and active sites of canonical calpains. Zygotic transcription of the gene was first detected at stage 10. It is expressed exclusively in the ventral circumblastoporal collar and the mesoderm-free zone at the most anterior tip of neural fold in late gastrulae and neurulae. In later stages, expression is only found in cement gland and proctodeum. It is also expressed in a tissue-specific manner. In adult tissues, various levels of expression were detected in brain, eye, heart, intestine, kidney, lung, stomach and testis, but not in liver, muscle, nerve, ovary, skin and spleen. Overexpression of wild-type XCL-2 suggests that this gene is involved in gastrulation movement and convergent extension during gastrulation and neurulation. Overexpression of a dominant-negative mutant caused a phenotype morphologically similar to, but histologically different from, that caused by overexpression of wild-type XCL-2. The mutant phenotype can be rescued by injection of wild-type XCL-2. These data suggest that XCL-2 plays an important role in convergent extension movements during embryogenesis in Xenopus laevis. |
| | |
Authors:
|
Y Cao; H Zhao; H Grunz |
Related Documents
:
|
9563913 - Regulation of ectodermal differentiation in xenopus laevis animal caps treated with tpa... 16150623 - Identification and expression of amphioxus beta-microseminoprotein (msp)-like gene enco... 21509893 - Zebrafish limb development is triggered by a retinoic acid signal during gastrulation. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Development, growth & differentiation Volume: 43 ISSN: 0012-1592 ISO Abbreviation: Dev. Growth Differ. Publication Date: 2001 Oct |
Date Detail:
|
Created Date: 2001-09-28 Completed Date: 2002-02-21 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0356504 Medline TA: Dev Growth Differ Country: Japan |
Other Details:
|
Languages: eng Pagination: 563-71 Citation Subset: IM |
Affiliation:
|
Department of Zoophysiology, University of Essen, 45117 Essen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Base Sequence Binding Sites Calpain / biosynthesis*, chemistry*, genetics*, metabolism, physiology* DNA, Complementary / metabolism Embryo, Nonmammalian / metabolism*, physiology Gastrula / metabolism Gene Library Genes, Dominant In Situ Hybridization Molecular Sequence Data Mutation Phenotype Plasmids / metabolism RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Time Factors Tissue Distribution Xenopus laevis / genetics*, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/RNA, Messenger; EC 3.4.22.-/Calpain; EC 3.4.22.-/XCL-2 calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Upregulation of cellular retinoic acid-binding protein I expression by ethanol.
Next Document: Ascidian Wnt-5 gene is involved in the morphogenetic movement of notochord cells.