Document Detail

X-ray microanalytic method for measurement of dry matter and elemental content of individual bacteria.
MedLine Citation:
PMID:  3911897     Owner:  NLM     Status:  MEDLINE    
A method for the determination of dry matter and elemental content of individual bacterial cells is described. The method is based on energy-dispersive X-ray microanalysis in a transmission electron microscope. A theory for area correction of intensity is developed. Escherichia coli in the late exponential phase of growth and early stationary phase (glucose limited) had an average dry matter content of 278 and 154 fg/cell, respectively. Of the elements detected, sodium, magnesium, phosphorus, sulphur, chlorine, potassium, and calcium together made up 15 to 17% of the dry matter content. A phosphorus content of 4.2 to 5.4% of the dry matter was found in these cells. Volume measurements of air-dried cells gave an average of 1.20 to 1.25 micron3. These results emphasize that dry matter content and elemental composition can be measured directly on single cells from complex microbial communities.
M Heldal; S Norland; O Tumyr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  50     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-02-28     Completed Date:  1986-02-28     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1251-7     Citation Subset:  IM    
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MeSH Terms
Electron Probe Microanalysis / methods
Elements / analysis*
Escherichia coli / analysis*,  growth & development,  ultrastructure
Microscopy, Electron
Reg. No./Substance:

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