Document Detail


X-ray microanalysis of ultrathin frozen and freeze-dried sections of human sperm cells.
MedLine Citation:
PMID:  988856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
X-ray microanalysis of air dried and ultrathin frozen and frozen dried sections of human sperm cells has indicated a large deviation of elemental composition between cells in a single sample and between samples. Analysis of air dried cells indicates a similar subcellular elemental distribution to that found in sectioned material. Sodium to potassium ratios are found to be similar in both preparations. Air drying is considered a valid method for the preparation of sperm cells for analysis.
Authors:
J A Chandler; S Battersby
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  107     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  55-65     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Electron Probe Microanalysis*
Freeze Drying
Frozen Sections*
Humans
Male
Microtomy*
Potassium / analysis
Sodium / analysis
Spermatozoa / analysis*,  ultrastructure
X-Rays
Chemical
Reg. No./Substance:
7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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