Document Detail


X-ray microanalysis of HeLa S3 cells. II. Analysis of elemental levels during the cell cycle.
MedLine Citation:
PMID:  6619208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HeLa S3 cells were synchronized using hydroxyurea. Cryoultramicrotomy and X-ray microanalysis were used to study changes occurring in concentrations of elements during the cell cycle of the synchronized cells. Three subcellular compartments were studied : cytoplasm, nucleus and nucleolus. Potassium concentrations showed little fluctuation in all of the cell compartments during the cell cycle. Sodium concentrations increased during S. and M phases, returning to lower levels in the G1 phase. Chlorine concentrations were highest during the S and G2 phases. At all stages of the cell cycle respective concentrations of potassium, sodium, sulphur and chlorine were similar in the cytoplasm and nucleus. Concentrations of phosphorus increased in the nucleus during S, G2 and M, and also showed fluctuations in the nucleolus during the cycle; these were not seen in the cytoplasm. In S, M and M/G1 sodium concentrations were highest in the nucleolus compared with the other compartments. In the cytoplasm these changes resulted in an increase in total monovalent cation concentration (i.e. sodium + potassium) during S, G2 and M, which returned to base levels after mitosis. This increase in monovalent cation concentration is due almost entirely to the increase in sodium, with little change occurring in the concentration of potassium.
Authors:
A Warley; J Stephen; A Hockaday; T C Appleton
Related Documents :
10570938 - Indomethacin-induced g1/s phase arrest of the plant cell cycle.
9732748 - A temperature-sensitive dna synthesis mutant isolated from the chinese hamster ovary ce...
730778 - Requirements of bhk cells for the exit from different quiescent states.
3108188 - Cell cycle kinetics responses of human stomach cancer cells to reduction in polyamine l...
24958878 - Fluid flows created by swimming bacteria drive self-organization in confined suspensions.
9724098 - Raralpha antagonist ro 41-5253 inhibits proliferation and induces apoptosis in breast-c...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  62     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  339-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Cell Nucleolus / analysis
Cell Nucleus / analysis
Chlorine / analysis
Cytoplasm / analysis
DNA / biosynthesis
Electron Probe Microanalysis
Hela Cells / analysis*,  cytology
Humans
Phosphorus / analysis
Potassium / analysis
Sodium / analysis
Sulfur / analysis
Time Factors
Chemical
Reg. No./Substance:
7440-09-7/Potassium; 7440-23-5/Sodium; 7704-34-9/Sulfur; 7723-14-0/Phosphorus; 7782-50-5/Chlorine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  X-ray microanalysis of proximal and distal tubule cells in the mouse kidney, and the influence of ca...
Next Document:  Isolation and characterization of invertebrate smooth septate junctions.