Document Detail

X-ray microanalysis of HeLa S3 cells. II. Analysis of elemental levels during the cell cycle.
MedLine Citation:
PMID:  6619208     Owner:  NLM     Status:  MEDLINE    
HeLa S3 cells were synchronized using hydroxyurea. Cryoultramicrotomy and X-ray microanalysis were used to study changes occurring in concentrations of elements during the cell cycle of the synchronized cells. Three subcellular compartments were studied : cytoplasm, nucleus and nucleolus. Potassium concentrations showed little fluctuation in all of the cell compartments during the cell cycle. Sodium concentrations increased during S. and M phases, returning to lower levels in the G1 phase. Chlorine concentrations were highest during the S and G2 phases. At all stages of the cell cycle respective concentrations of potassium, sodium, sulphur and chlorine were similar in the cytoplasm and nucleus. Concentrations of phosphorus increased in the nucleus during S, G2 and M, and also showed fluctuations in the nucleolus during the cycle; these were not seen in the cytoplasm. In S, M and M/G1 sodium concentrations were highest in the nucleolus compared with the other compartments. In the cytoplasm these changes resulted in an increase in total monovalent cation concentration (i.e. sodium + potassium) during S, G2 and M, which returned to base levels after mitosis. This increase in monovalent cation concentration is due almost entirely to the increase in sodium, with little change occurring in the concentration of potassium.
A Warley; J Stephen; A Hockaday; T C Appleton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  62     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  339-50     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Nucleolus / analysis
Cell Nucleus / analysis
Chlorine / analysis
Cytoplasm / analysis
DNA / biosynthesis
Electron Probe Microanalysis
Hela Cells / analysis*,  cytology
Phosphorus / analysis
Potassium / analysis
Sodium / analysis
Sulfur / analysis
Time Factors
Reg. No./Substance:
7440-09-7/Potassium; 7440-23-5/Sodium; 7704-34-9/Sulfur; 7723-14-0/Phosphorus; 7782-50-5/Chlorine; 9007-49-2/DNA

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