| X-ray imaging of differential vascular density in MMP-9-/-, PAR-1-/+, hyperhomocysteinemic (CBS-/+) and diabetic (Ins2-/+) mice. | |
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MedLine Citation:
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PMID: 20839901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although protease activated receptor-1 (PAR-1) and matrix metalloproteinase-9 (MMP-9) play significant role in vascular remodelling in hyperhomocysteinemia (HHcy due to cystathionine beta synthase deficiency, CBS-/+) and diabetes, mechanism remains nebulous. We hypothesized that differential vascular density and remodelling in different vascular beds in HHcy and diabetes were responsible for an adaptive metabolic homeostasis during the pathogenesis. To test this hypothesis, vascular density in mice lacking PAR-1, MMP-9, CBS and Insulin-2 gene mutant (Ins2-/+, Akita) was measured and compared with wild type (WT, C57BL/6J) mice. The vascular density was detected by x-ray angiography using KODAK 4000 MM image station, using barium sulphate as contrasting agent. The % vascular density in the hearts of WT, CBS-/+ (HHcy), MMP-9-/-, PAR-1-/+ and Ins2-/+ (type-1 diabetes) was 100 ± 2.8, 85 ± 3.3, 90 ± 3.3, 95 ± 3.8 and 73 ± 1.7, respectively. The vascular density in CBS-/+ and Akita hearts decreased while it was increased in lungs of CBS-/+ and MMP-9-/-.There was decreased vascular density in liver and kidney of Akita mice. Vascular density in brain, kidney and mesentery was decreased in CBS-/+ mice. These findings support the notation that metabolic derangement in diabetes and HHcy causes the chronic decline and/or rarefaction in vascular density. |
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Authors:
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S Givvimani; U Sen; N Tyagi; C Munjal; S C Tyagi |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-14 |
Journal Detail:
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Title: Archives of physiology and biochemistry Volume: 117 ISSN: 1744-4160 ISO Abbreviation: Arch. Physiol. Biochem. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-06 Completed Date: 2011-04-13 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 9510153 Medline TA: Arch Physiol Biochem Country: England |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, University of Louisville School of Medicine, KY 40202, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiography Animals Barium Sulfate / analysis Blood Vessels* / pathology Brain / blood supply Cystathionine beta-Synthase / deficiency, genetics Diabetes Mellitus, Type 2* / genetics, metabolism, physiopathology Disease Models, Animal Hyperhomocysteinemia* / genetics, metabolism, physiopathology Matrix Metalloproteinase 9* / deficiency, genetics Mice Mice, Inbred C57BL Mice, Transgenic Organ Size / genetics Receptor, PAR-1* / deficiency, genetics Renal Circulation Splanchnic Circulation X-Rays |
| Grant Support | |
ID/Acronym/Agency:
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HL-71010/HL/NHLBI NIH HHS; HL-74185/HL/NHLBI NIH HHS; HL-88012/HL/NHLBI NIH HHS; NS-51568/NS/NINDS NIH HHS; R01 HL074185-09/HL/NHLBI NIH HHS; R01 HL088012-03/HL/NHLBI NIH HHS; R01 HL108621-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptor, PAR-1; 7727-43-7/Barium Sulfate; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 4.2.1.22/Cystathionine beta-Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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