| X-ray diffraction to determine the thickness of raft and nonraft bilayers. | |
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MedLine Citation:
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PMID: 18214383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Low-angle X-ray diffraction is a powerful method to analyze the structure of membrane bilayers. Specifically, the technique can be used to determine accurately the thickness of fully hydrated bilayers. Herein details are presented showing how this technique can measure the difference in thickness of bilayers in detergent-resistant membranes and detergent-soluble membranes extracted from model systems known to contain both raft and nonraft domains. The observed thickness difference may be critical in the sorting of transmembrane proteins between raft and nonraft bilayers. |
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Authors:
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Thomas J McIntosh |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 398 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2008-01-24 Completed Date: 2008-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 221-30 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cholesterol
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chemistry Detergents Electrons Hydrocarbons / chemistry Lipid Bilayers / chemistry* Membrane Microdomains / chemistry* Membrane Proteins / analysis, chemistry Phosphatidylcholines / chemistry Solubility Sphingomyelins / chemistry X-Ray Diffraction / methods* |
| Grant Support | |
ID/Acronym/Agency:
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GM27278/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Detergents; 0/Hydrocarbons; 0/Lipid Bilayers; 0/Membrane Proteins; 0/Phosphatidylcholines; 0/Sphingomyelins; 10015-85-7/1,2-oleoylphosphatidylcholine; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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