Document Detail


X-ray diffraction to determine the thickness of raft and nonraft bilayers.
MedLine Citation:
PMID:  18214383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Low-angle X-ray diffraction is a powerful method to analyze the structure of membrane bilayers. Specifically, the technique can be used to determine accurately the thickness of fully hydrated bilayers. Herein details are presented showing how this technique can measure the difference in thickness of bilayers in detergent-resistant membranes and detergent-soluble membranes extracted from model systems known to contain both raft and nonraft domains. The observed thickness difference may be critical in the sorting of transmembrane proteins between raft and nonraft bilayers.
Authors:
Thomas J McIntosh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  398     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  221-30     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Cholesterol / chemistry
Detergents
Electrons
Hydrocarbons / chemistry
Lipid Bilayers / chemistry*
Membrane Microdomains / chemistry*
Membrane Proteins / analysis,  chemistry
Phosphatidylcholines / chemistry
Solubility
Sphingomyelins / chemistry
X-Ray Diffraction / methods*
Grant Support
ID/Acronym/Agency:
GM27278/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Detergents; 0/Hydrocarbons; 0/Lipid Bilayers; 0/Membrane Proteins; 0/Phosphatidylcholines; 0/Sphingomyelins; 10015-85-7/1,2-oleoylphosphatidylcholine; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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