Document Detail

X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi.
MedLine Citation:
PMID:  16011007     Owner:  NLM     Status:  MEDLINE    
Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA3 staining reveals that these silver positive telomeres are the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA.
A Criniti; G Simonazzi; S Cassanelli; M Ferrari; D Bizzaro; G C Manicardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetica     Volume:  124     ISSN:  0016-6707     ISO Abbreviation:  Genetica     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-13     Completed Date:  2005-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370740     Medline TA:  Genetica     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
Dipartimento di Scienze Agrarie, Università di Modena e Reggio Emilia, Reggio Emilia, Italia.
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MeSH Terms
Aphids / genetics*
DNA, Ribosomal / analysis
Heterochromatin / chemistry*
In Situ Hybridization, Fluorescence
X Chromosome / chemistry
Reg. No./Substance:
0/DNA, Ribosomal; 0/Heterochromatin

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