Document Detail


X-irradiation--induced changes in the progression of type B spermatogonia and preleptotene spermatocytes.
MedLine Citation:
PMID:  11144224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In response to induced DNA damage, proliferating cells arrest in their cell cycle or go into apoptosis. Ionizing radiation is known to induce degeneration of mammalian male germ cells. The effects on cell-cycle progression, however, have not been thoroughly studied due to lack of methods for identifying effects on a particular cell-cycle phase of a specific germ cell type. In this study, we have utilized the technique for isolation of defined segments of seminiferous tubules to examine the cell-cycle progression of irradiated rat mitotic (type B spermatogonia) and meiotic (preleptotene spermatocytes) G1/S cells. Cells irradiated as type B spermatogonia in mitotic S phase showed a small delay in progression through meiosis. Thus, it seems that transient arrest in the progression can occur in the otherwise strictly regulated progression of germ cells in the seminiferous epithelium. Contrary to the arrest observed in type B spermatogonia and in previous studies on somatic cells, X-irradiation did not result in a G1 delay in meiotic cells. This lack of arrest occurred despite the presence of unrepaired DNA damage that was measured when the cells had progressed through the two meiotic divisions.
Authors:
A West; J Lähdetie
Related Documents :
4043394 - The 25 kda protein kinase inhibitor present in liver cell is absent in fast growing htc...
8041894 - The molecular basis for cell cycle delays following ionizing radiation: a review.
824054 - Hydroxyurea: use in determining the duration of g2 in tetrahymena.
10551774 - Transfection of an inducible p16/cdkn2a construct mediates reversible growth inhibition...
19946214 - Sex steroid-mediated reprogramming of vascular smooth muscle cells to stem cells and ne...
12202834 - Identification of a potential ejaculation generator in the spinal cord.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  58     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2000-12-28     Completed Date:  2001-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
Affiliation:
Turku Graduate School of Biomedical Sciences, University of Turku, Finland. annwes@utu.fi
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Death
G1 Phase
G2 Phase
Male
Meiosis
Mitosis
Rats
Rats, Sprague-Dawley
S Phase
Spermatocytes / cytology,  radiation effects*
Spermatogonia / cytology,  radiation effects*
Testis / cytology,  radiation effects

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Colocalization of CaM KII and MAP kinase on architectural elements of the mouse egg: potentiation of...
Next Document:  Novel sperm-binding proteins of epididymal origin contain four fibronectin type II-modules.