Document Detail

X-irradiation--induced changes in the progression of type B spermatogonia and preleptotene spermatocytes.
MedLine Citation:
PMID:  11144224     Owner:  NLM     Status:  MEDLINE    
In response to induced DNA damage, proliferating cells arrest in their cell cycle or go into apoptosis. Ionizing radiation is known to induce degeneration of mammalian male germ cells. The effects on cell-cycle progression, however, have not been thoroughly studied due to lack of methods for identifying effects on a particular cell-cycle phase of a specific germ cell type. In this study, we have utilized the technique for isolation of defined segments of seminiferous tubules to examine the cell-cycle progression of irradiated rat mitotic (type B spermatogonia) and meiotic (preleptotene spermatocytes) G1/S cells. Cells irradiated as type B spermatogonia in mitotic S phase showed a small delay in progression through meiosis. Thus, it seems that transient arrest in the progression can occur in the otherwise strictly regulated progression of germ cells in the seminiferous epithelium. Contrary to the arrest observed in type B spermatogonia and in previous studies on somatic cells, X-irradiation did not result in a G1 delay in meiotic cells. This lack of arrest occurred despite the presence of unrepaired DNA damage that was measured when the cells had progressed through the two meiotic divisions.
A West; J Lähdetie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  58     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2000-12-28     Completed Date:  2001-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
Turku Graduate School of Biomedical Sciences, University of Turku, Finland.
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MeSH Terms
Cell Death
G1 Phase
G2 Phase
Rats, Sprague-Dawley
S Phase
Spermatocytes / cytology,  radiation effects*
Spermatogonia / cytology,  radiation effects*
Testis / cytology,  radiation effects

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