Document Detail

X-cells in pseudotumors of yellowfin goby Acanthogobius flavimanus: a protistan organism distinct from that in flathead flounder Hippoglossoides dubius.
MedLine Citation:
PMID:  19593933     Owner:  NLM     Status:  MEDLINE    
Yellowfin goby Acanthogobius flavimanus affected with X-cell pseudotumors were sampled from a river estuary in Tokyo Bay, Japan. We amplified the gene for small subunit ribosomal RNA (18S rRNA) of X-cells of the goby with PCR using universal primers. The gene that we obtained (DDBJ Accession no. AB451874) showed 91% sequence identity to that of the X-cells of the flathead flounder Hippoglossoides dubius. With in situ hybridization, the probes specific for the gene that we obtained hybridized with the goby X-cells but not with the flounder X-cells, whereas probes for the 18S rRNA gene of flounder X-cells hybridized with the flounder X-cells but not with goby X-cells. These findings indicate that, although the X-cells found in the goby are closely related to the protist found in flounder, the two are clearly distinct organisms.
Satoshi Miwa; Takashi Kamaishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  85     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-07-14     Completed Date:  2009-08-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  53-7     Citation Subset:  IM    
Inland Station, National Research Institute of Aquaculture, Tamaki, Mie 519-0423, Japan.
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MeSH Terms
Eukaryota / classification*,  genetics*
Fish Diseases / parasitology
Flounder / parasitology*
Granuloma, Plasma Cell / parasitology,  pathology,  veterinary*
Molecular Sequence Data
Perciformes / parasitology*
Protozoan Infections, Animal / parasitology*
RNA, Ribosomal, 18S / genetics
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/RNA, Ribosomal, 18S

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