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THE WST SURVIVAL ASSAY: AN EASY AND RELIABLE METHOD TO SCREEN RADIATION-SENSITIVE INDIVIDUALS.
MedLine Citation:
PMID:  21183542     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An easy, fast and reliable method was developed to screen hundreds of Epstein-Barr virus-transformed cell lines (lymphoblastoid cell lines, LCLs) for radiation sensitivity that were generated from lymphocytes isolated from young lung cancer patients. The WST-1 test explores the metabolic activity of the mitochondria as an indicator for the vital status of cells. Cell proliferation as well as indirect cell death can be quantified by this method on a large scale in microtiter plates. Cell survival was measured at 24- and 48-h post-irradiation with 10 Gy ((137)Cs source) by the WST-1 assay and Trypan blue staining. To set up the experimental screening conditions and to establish a positive and a negative control, an ATM-mutated cell line from a radiation-sensitive ATM patient and an ATM proficient cell line from a healthy brother were compared. An optimal differentiation between the two cell lines was demonstrated for 10 Gy and 24- and 48-h cell growth after irradiation. Upon screening 120 LCLs of young lung cancer patients under these conditions, 5 of them were found to be radiation sensitive to a high degree of statistical significance. The results have been confirmed by a second laboratory by means of Trypan blue testing. The WST-1 test represents an efficient and reliable method by means of screening for radiation-sensitive cell lines.
Authors:
A Guertler; A Kraemer; U Roessler; S Hornhardt; U Kulka; S Moertl; A A Friedl; T Illig; E Wichmann; M Gomolka
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-23
Journal Detail:
Title:  Radiation protection dosimetry     Volume:  -     ISSN:  1742-3406     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109958     Medline TA:  Radiat Prot Dosimetry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department SG Radiation Protection and Health, Federal Office for Radiation Protection, Oberschleissheim, Germany.
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