Document Detail


Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets.
MedLine Citation:
PMID:  17381626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The wound-healing applications of platelet (PLT)-derived cytokines, proteins, and membranes is accepted but continues to be investigated. In this study, it is demonstrated that stabilized freeze-dried PLTs prepared from outdated PLTs (FDPOs) accelerate wound healing and form tube structures as well as stabilized indated freeze-dried PLTs (FDPIs) and room-temperature fresh PLTs (RT-PLTs). STUDY DESIGN AND METHODS: Experiments were designed to compare in vitro and in vivo wound-healing properties of FDPI, FDPO, and RT-PLT preparations. The concentration of PLT-derived growth factor (PDGF)-betabeta and transforming growth factor (TGF)-beta1 was determined, and the abilities of FDPIs, FDPOs and RT-PLTs to induce endothelial cell proliferation and promote endothelial cell tube formation (cells formed solid spouts connecting neighboring cells to form tube structures) were observed. Wound-healing characteristics were measured by surgically inducing 1-cm(2), full-thickness wounds on db/db mice (n = 10 per group). The wounds were treated with single or multiple doses of FDPIs and FDPOs. Wound closure rate was determined, and histology samples were evaluated for cellular makeup. RESULTS: FDPOs retained the same levels of PDGF-betabeta and TGF-beta1 and were able to promote endothelial cell proliferation and tube formation in vitro as well as FDPIs or RT-PLTs. Multiple applications of FDPO accelerated wound closure and enhanced reepithelialization when compared to untreated wounds in db/db mice. CONCLUSION: FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT-PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis.
Authors:
Ruth Sum; Sarah Hager; Giorgio Pietramaggiori; Dennis P Orgill; Josh Dee; Alan Rudolph; Cindy Orser; G Michael Fitzpatrick; David Ho
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Transfusion     Volume:  47     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-26     Completed Date:  2007-09-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  672-9     Citation Subset:  IM    
Affiliation:
From Adlyfe Inc., and Cellphire Inc., Rockville, Maryland 22101, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Platelets / drug effects,  metabolism,  physiology*
Blood Preservation / methods
Cell Proliferation
Cells, Cultured
Diabetes Mellitus, Type 2 / blood,  physiopathology
Freeze Drying / methods*
Humans
Male
Mice
Platelet Transfusion / methods
Platelet-Derived Growth Factor / metabolism
Transforming Growth Factor beta1 / metabolism
Trehalose / pharmacology*
Wound Healing*
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor; 0/Transforming Growth Factor beta1; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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