Document Detail

Wound-healing gene family expression differences between fetal and foreskin cells used for bioengineered skin substitutes.
MedLine Citation:
PMID:  18638304     Owner:  NLM     Status:  MEDLINE    
For tissue engineering, several cell types and tissues have been proposed as starting material. Allogenic skin products available for therapeutic usage are mostly developed with cell culture and with foreskin tissue of young individuals. Fetal skin cells offer a valuable solution for effective and safe tissue engineering for wounds due to their rapid growth and simple cell culture. By selecting families of genes that have been reported to be implicated in wound repair and particularly for scarless fetal wound healing including transforming growth factor-beta (TGF-beta) superfamily, extracellular matrix, and nerve/angiogenesis growth factors, we have analyzed differences in their expression between fetal skin and foreskin cells, and the same passages. Of the five TGF-beta superfamily genes analyzed by real-time reverse transcription-polymerase chain reaction, three were found to be significantly different with sixfold up-regulated for TGF-beta2, and 3.8-fold for BMP-6 in fetal cells, whereas GDF-10 was 11.8-fold down-regulated. For nerve growth factors, midkine was 36-fold down-regulated in fetal cells, and pleiotrophin was 4.76-fold up-regulated. We propose that fetal cells present technical and therapeutic advantages compared to foreskin cells for effective cell-based therapy for wound management, and overall differences in gene expression could contribute to the degree of efficiency seen in clinical use with these cells.
Nathalie Hirt-Burri; Corinne Scaletta; Stefan Gerber; Dominique P Pioletti; Lee Ann Applegate
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial organs     Volume:  32     ISSN:  1525-1594     ISO Abbreviation:  Artif Organs     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-21     Completed Date:  2008-08-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  509-18     Citation Subset:  IM    
Department of Pediatric Surgery, University Hospital, CHUV, Lausanne, Switzerland.
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MeSH Terms
Bone Morphogenetic Protein 3
Bone Morphogenetic Protein 6
Bone Morphogenetic Proteins / genetics
Carrier Proteins / genetics
Cell Line
Cell Transplantation
Cytokines / genetics
Fibroblasts / cytology,  metabolism
Foreskin / cytology*,  metabolism
Gene Expression Regulation
Growth Differentiation Factor 10
Multigene Family*
Nerve Growth Factors / genetics
RNA / genetics
Reverse Transcriptase Polymerase Chain Reaction
Skin / cytology*,  embryology,  metabolism
Skin Transplantation
Skin, Artificial
Tissue Culture Techniques
Tissue Engineering*
Transforming Growth Factor beta2 / genetics
Wound Healing / genetics*
Reg. No./Substance:
0/BMP3 protein, human; 0/BMP6 protein, human; 0/Bone Morphogenetic Protein 3; 0/Bone Morphogenetic Protein 6; 0/Bone Morphogenetic Proteins; 0/Carrier Proteins; 0/Cytokines; 0/GDF10 protein, human; 0/Growth Differentiation Factor 10; 0/Nerve Growth Factors; 0/Transforming Growth Factor beta2; 134034-50-7/pleiotrophin; 137497-38-2/midkine; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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