Document Detail


Wolbachia in two populations of Melittobia digitata Dahms (Hymenoptera: Eulophidae).
MedLine Citation:
PMID:  19169549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated two populations of Melittobia digitata Dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of Wolbachia infection. The first population, from Xalapa, Mexico, was originally collected from and reared on Mexican fruit fly pupae, Anastrepha ludens Loew (Diptera: Tephritidae); the other, from Athens, Georgia, was collected from and reared on prepupae of mud dauber wasps, Trypoxylon politum Say (Hymenoptera: Crabronidae). PCR studies of the ITS2 region corroborated that both parasitoid populations were the same species; this potentially provides a useful molecular taxonomic profile since females of Melittobia species are superficially similar. Amplification of the Wolbachia surface protein gene (wsp) confirmed the presence of this endosymbiont in both populations. Sequencing revealed that the Wolbachia harbored in both populations exhibited a wsp belonging to a unique subgroup (denoted here as Dig) within the B-supergroup of known wsp genes. This new subgroup of wsp may either belong to a different strain of Wolbachia from those previously found to infect Melittobia or may be the result of a recombination event. In either case, known hosts of Wolbachia with a wsp of this subgroup are only distantly related taxonomically. Reasons are advanced as to why Melittobia - an easily reared and managed parasitoid - holds promise as an instructive model organism of Wolbachia infection amenable to the investigation of Wolbachia strains among its diverse hosts.
Authors:
Claudia S Copeland; Robert W Matthews; Jorge M González; Martin Aluja; John Sivinski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Neotropical entomology     Volume:  37     ISSN:  1519-566X     ISO Abbreviation:  Neotrop. Entomol.     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101189728     Medline TA:  Neotrop Entomol     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  633-40     Citation Subset:  IM    
Affiliation:
USDA, ARS, CMAVE, 1700 SW 23rd Dr., Gainesville, FL 32608, USA. cclaudia@bioinf.uni-leipzig.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Hymenoptera / microbiology*
Male
Polymerase Chain Reaction
Wolbachia / isolation & purification*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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